ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Comparison of cysteine string protein (Csp) and mutant alpha-SNAP overexpression reveals a role for csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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Who cites it
38 citing papers in PubMed, 111 citations in OpenAlex.
- Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.The Biochemical journal · 2024Article
- Vesicle Fusion as a Target Process for the Action of Sphingosine and Its Derived Drugs.International journal of molecular sciences · 2022Review
- Autosomal dominant neuronal ceroid lipofuscinosis: Clinical features and molecular basis.Clinical genetics · 2021Review
- GPCR regulation of secretion.Pharmacology & therapeutics · 2018Review
- Emerging evidence for the modulation of exocytosis by signalling lipids.FEBS letters · 2018Review
- Fusion pores and their control of neurotransmitter and hormone release.The Journal of general physiology · 2017Review
- DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins.The EMBO journal · 2016Article
- Cysteine string protein (CSP) and its role in preventing neurodegeneration.Seminars in cell & developmental biology · 2015Review
- Genome-wide features of neuroendocrine regulation in Drosophila by the basic helix-loop-helix transcription factor DIMMED.Nucleic acids research · 2015Article
- Article
- NMNATs, evolutionarily conserved neuronal maintenance factors.Trends in neurosciences · 2013Review
- Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot.EMBO reports · 2013Article
- The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.Plant molecular biology · 2012Article
- Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster.The Journal of comparative neurology · 2010Article
- Enhanced dense core granule function and adrenal hypersecretion in a mouse model of Rett syndrome.The European journal of neuroscience · 2009Article
- Functional role of J domain of cysteine string protein in Ca2+-dependent secretion from acinar cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Cysteine string protein promotes proteasomal degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) by increasing its interaction with the C terminus of Hsp70-interacting protein and promoting CFTR ubiquitylation.The Journal of biological chemistry · 2009Article
- The fusion pores of Ca2+ -triggered exocytosis.Nature structural & molecular biology · 2008Review
- Temperature dependence of fusion kinetics and fusion pores in Ca2+-triggered exocytosis from PC12 cells.The Journal of general physiology · 2008Article
- Controlled on-chip stimulation of quantal catecholamine release from chromaffin cells using photolysis of caged Ca2+ on transparent indium-tin-oxide microchip electrodes.Lab on a chip · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Assembly of the SNARE complex and its disassembly caused by the action of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) and NSF is crucial for the maintenance of vesicular traffic, including fusion of regulated exocytotic vesicles. Various other proteins may also have important roles in the processes leading to membrane fusion via interaction with the SNARE proteins, including the secretory vesicle cysteine string protein (Csp). Here we have examined the effect of overexpression of a dominant negative alpha-SNAP mutant or Csp on exocytosis of dense-core granules in single chromaffin cells monitored using amperometry to detect released catecholamine. Exocytosis of trans-Golgi network (TGN)-derived dense-core granules was substantially inhibited by expression of alpha-SNAP(L294A). The amplitude and characteristics of the individual release events were unaffected by expression of alpha-SNAP(L294A), consistent with an essential role for alpha-SNAP in early steps of priming but not in the fusion process. In contrast, Csp overexpression, which also inhibited the extent of exocytosis, also modified the kinetics of the individual release events seen as an increase in the rise time and a broadening of the residual amperometric spikes in Csp-transfected cells. These results suggest that unlike alpha-SNAP, Csp plays a key role in the protein interactions close to the fusion process or fusion pore opening during Ca(2+)-regulated exocytosis.
Indexed as
Identifiers
10662817PMC6772362W1798761425What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.