ArticleThe Journal of cell biology2000
Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 243 citations in OpenAlex.
- 3D printing cytoskeletal networks: ROS-induced filament severing leads to surge in actin polymerization.bioRxiv : the preprint server for biology · 2025Article
- TPM4 condensates glycolytic enzymes and facilitates actin reorganization under hyperosmotic stress.Cell discovery · 2024Article
- Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023Review
- CryoET shows cofilactin filaments inside the microtubule lumen.EMBO reports · 2023Article
- CryoET shows cofilactin filaments inside the microtubule lumen.bioRxiv : the preprint server for biology · 2023Article
- The Actin Regulators Involved in the Function and Related Diseases of Lymphocytes.Frontiers in immunology · 2022Review
- Distinct forms of the actin cross-linking protein α-actinin support macropinosome internalization and trafficking.Molecular biology of the cell · 2021Article
- Serum levels of cytoskeleton remodeling proteins and their mRNA expression in tumor tissue of metastatic laryngeal and hypopharyngeal cancers.Molecular biology reports · 2021Article
- Cofilin is required for polarization of tension in stress fiber networks during migration.Journal of cell science · 2020Article
- Serotonin 5-HTCommunications biology · 2020Article
- Optogenetic control of cofilin and αTAT in living cells using Z-lock.Nature chemical biology · 2019Article
- Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation.Science (New York, N.Y.) · 2018Article
- Rho A Regulates Epidermal Growth Factor-Induced Human Osteosarcoma MG63 Cell Migration.International journal of molecular sciences · 2018Article
- Role of Bone Morphogenetic Protein 7 (BMP7) in the Modulation of Corneal Stromal and Epithelial Cell Functions.International journal of molecular sciences · 2018Article
- Inverted formins: A subfamily of atypical formins.Cytoskeleton (Hoboken, N.J.) · 2017Review
- NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin.EBioMedicine · 2017Article
- Integrated Analysis of Intracellular Dynamics of MenaINV Cancer Cells in a 3D Matrix.Biophysical journal · 2017Article
- Cofilin Activation Is Temporally Associated with the Cessation of Growth in the Developing Hippocampus.Cerebral cortex (New York, N.Y. : 1991) · 2017Article
- Cofilin-1 and Other ADF/Cofilin Superfamily Members in Human Malignant Cells.International journal of molecular sciences · 2016Review
- The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior.Scientific reports · 2016Article
43 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Stimulation of metastatic MTLn3 cells with epidermal growth factor (EGF) causes a rapid and transient increase in actin nucleation activity resulting from the appearance of free barbed ends at the extreme leading edge of extending lamellipods. To investigate the role of cofilin in EGF-stimulated actin polymerization and lamellipod extension in MTLn3 cells, we examined in detail the temporal and spatial distribution of cofilin relative to free barbed ends and characterized the actin dynamics by measuring the changes in the number of actin filaments. EGF stimulation triggers a transient increase in cofilin in the leading edge near the membrane, which is precisely cotemporal with the appearance of free barbed ends there. A deoxyribonuclease I binding assay shows that the number of filaments per cell increases by 1.5-fold after EGF stimulation. Detection of pointed ends in situ using deoxyribonuclease I binding demonstrates that this increase in the number of pointed ends is confined to the leading edge compartment, and does not occur within stress fibers or in the general cytoplasm. Using a light microscope severing assay, cofilin's severing activity was observed directly in cell extracts and shown to be activated after stimulation of the cells with EGF. Microinjection of function-blocking antibodies against cofilin inhibits the appearance of free barbed ends at the leading edge and lamellipod protrusion after EGF stimulation. These results support a model in which EGF stimulation recruits cofilin to the leading edge where its severing activity is activated, leading to the generation of short actin filaments with free barbed ends that participate in the nucleation of actin polymerization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.