ArticleThe Journal of cell biology2000
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 180 citations in OpenAlex.
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- Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging.BMC plant biology · 2024Article
- A View into Seed Autophagy: From Development to Environmental Responses.Plants (Basel, Switzerland) · 2022Article
- Specialized endoplasmic reticulum-derived vesicles in plants: Functional diversity, evolution, and biotechnological exploitation.Journal of integrative plant biology · 2022Review
- Coping with Abiotic Stress in Plants-An Endomembrane Trafficking Perspective.Plants (Basel, Switzerland) · 2022Review
- Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana.Nature communications · 2021Article
- Cytosolic GLUTAMINE SYNTHETASE1;1 Modulates Metabolism and Chloroplast Development in Roots.Plant physiology · 2020Article
- Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants.Communications biology · 2020Article
- Interaction between VPS35 and RABG3f is necessary as a checkpoint to control fusion of late compartments with the vacuole.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Alternative Oxidase Capacity of Mitochondria in Microsporophylls May Function in Cycad Thermogenesis.Plant physiology · 2019Article
- Insights on the Proteases Involved in Barley and Wheat Grain Germination.International journal of molecular sciences · 2019Review
- The role of KDEL-tailed cysteine endopeptidases of Arabidopsis (AtCEP2 and AtCEP1) in root development.PloS one · 2018Article
- The karrikin receptor KAI2 promotes drought resistance in Arabidopsis thaliana.PLoS genetics · 2017Article
- Article
- Characterization of Shikonin Derivative Secretion in Lithospermum erythrorhizon Hairy Roots as a Model of Lipid-Soluble Metabolite Secretion from Plants.Frontiers in plant science · 2016Article
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- Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis.Plant cell reports · 2015Article
- Silencing of the CaCP gene delays salt- and osmotic-induced leaf senescence in Capsicum annuum L.International journal of molecular sciences · 2014Article
- Ex vivo processing for maturation of Arabidopsis KDEL-tailed cysteine endopeptidase 2 (AtCEP2) pro-enzyme and its storage in endoplasmic reticulum derived organelles.Plant molecular biology · 2014Article
- Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco.The Plant cell · 2014Article
11 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A vacuolar cysteine proteinase, designated SH-EP, is expressed in the cotyledon of germinated Vigna mungo seeds and is responsible for the degradation of storage proteins. SH-EP is a characteristic vacuolar proteinase possessing a COOH-terminal endoplasmic reticulum (ER) retention sequence, KDEL. In this work, immunocytochemical analysis of the cotyledon cells of germinated V. mungo seeds was performed using seven kinds of antibodies to identify the intracellular transport pathway of SH-EP from ER to protein storage vacuoles. A proform of SH-EP synthesized in ER accumulated at the edge or middle region of ER where the transport vesicle was formed. The vesicle containing a large amount of proSH-EP, termed KV, budded off from ER, bypassed the Golgi complex, and was sorted to protein storage vacuoles. This massive transport of SH-EP via KV was thought to mediate dynamic protein mobilization in the cotyledon cells of germinated seeds. We discuss the possibilities that the KDEL sequence of KDEL-tailed vacuolar cysteine proteinases function as an accumulation signal at ER, and that the mass transport of the proteinases by ER-derived KV-like vesicle is involved in the protein mobilization of plants.
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