ArticleThe Journal of cell biology2000
Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 141 citations in OpenAlex.
- Germ granule-mediated mRNA storage and translational control.RNA biology · 2025Review
- The mRNA dynamics underpinning translational control mechanisms of Drosophila melanogaster oogenesis.Biochemical Society transactions · 2024Review
- Exosomal YB-1 facilitates ovarian restoration by MALAT1/miR-211-5p/FOXOCell biology and toxicology · 2024Article
- P-body-like condensates in the germline.Seminars in cell & developmental biology · 2024Review
- Article
- Mechanisms and Regulation of RNA Condensation in RNP Granule Formation.Trends in biochemical sciences · 2020Review
- Article
- Review
- Translational repression of theRNA (New York, N.Y.) · 2017Article
- Evolutionary coincidence of adaptive changes in exuperantia and the emergence of bicoid in Cyclorrhapha (Diptera).Development genes and evolution · 2017Article
- The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.Nature structural & molecular biology · 2016Article
- Keeping a lid on nodal: transcriptional and translational repression of nodal signalling.Open biology · 2016Review
- Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers.Developmental biology · 2015Article
- Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions.Nucleus (Austin, Tex.) · 2014Article
- Article
- mRNA localization and translational control in Drosophila oogenesis.Cold Spring Harbor perspectives in biology · 2012Review
- Assembly of mRNA-protein complexes for directional mRNA transport in eukaryotes--an overview.Current protein & peptide science · 2012Review
- Drosophila Syncrip binds the gurken mRNA localisation signal and regulates localised transcripts during axis specification.Biology open · 2012Article
- Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR.RNA (New York, N.Y.) · 2012Article
- New insights into the regulation of RNP granule assembly in oocytes.International review of cell and molecular biology · 2012Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Localization of bicoid (bcd) mRNA to the anterior and oskar (osk) mRNA to the posterior of the Drosophila oocyte is critical for embryonic patterning. Previous genetic studies implicated exuperantia (exu) in bcd mRNA localization, but its role in this process is not understood. We have biochemically isolated Exu and show that it is part of a large RNase-sensitive complex that contains at least seven other proteins. One of these proteins was identified as the cold shock domain RNA-binding protein Ypsilon Schachtel (Yps), which we show binds directly to Exu and colocalizes with Exu in both the oocyte and nurse cells of the Drosophila egg chamber. Surprisingly, the Exu-Yps complex contains osk mRNA. This biochemical result led us to reexamine the role of Exu in the localization of osk mRNA. We discovered that exu-null mutants are defective in osk mRNA localization in both nurse cells and the oocyte. Furthermore, both Exu/Yps particles and osk mRNA follow a similar temporal pattern of localization in which they transiently accumulate at the oocyte anterior and subsequently localize to the posterior pole. We propose that Exu is a core component of a large protein complex involved in localizing mRNAs both within nurse cells and the developing oocyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.