ArticleThe Biochemical journal2000
Endoproteolytic processing of integrin pro-alpha subunits involves the redundant function of furin and proprotein convertase (PC) 5A, but not paired basic amino acid converting enzyme (PACE) 4, PC5B or PC7.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 101 citations in OpenAlex.
- Extracellular Matrix of Echinoderms.Marine drugs · 2023Review
- Allele-Specific Epigenetic Regulation ofCells · 2023Article
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- Proprotein Convertases and the Complement System.Frontiers in immunology · 2022Review
- Short linear motif candidates in the cell entry system used by SARS-CoV-2 and their potential therapeutic implications.Science signaling · 2021Review
- Molecular evolution of PCSK family: Analysis of natural selection rate and gene loss.PloS one · 2021Article
- Selective inhibition of N-linked glycosylation impairs receptor tyrosine kinase processing.Disease models & mechanisms · 2019Article
- Influence of a Coronary Artery Disease-Associated Genetic Variant on FURIN Expression and Effect of Furin on Macrophage Behavior.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.Developmental cell · 2017Article
- A Golgi-localized pool of the mitotic checkpoint component Mad1 controls integrin secretion and cell migration.Current biology : CB · 2014Article
- Article
- Proprotein convertases play an important role in regulating PKGI endoproteolytic cleavage and nuclear transport.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Enhanced UV-induced skin carcinogenesis in transgenic mice overexpressing proprotein convertases.Neoplasia (New York, N.Y.) · 2013Article
- Gain of glycosylation in integrin α3 causes lung disease and nephrotic syndrome.The Journal of clinical investigation · 2012Article
- Laser-capture microdissection and transcriptional profiling of the dorsomedial nucleus of the hypothalamus.The Journal of comparative neurology · 2012Article
- Transgenic overexpression of the proprotein convertase furin enhances skin tumor growth.Neoplasia (New York, N.Y.) · 2012Article
- Embryo implantation is closely associated with dynamic expression of proprotein convertase 5/6 in the rabbit uterus.Reproductive biology and endocrinology : RB&E · 2011Article
- Proprotein convertase inhibition results in decreased skin cell proliferation, tumorigenesis, and metastasis.Neoplasia (New York, N.Y.) · 2010Article
- Subtilisin-like proprotein convertase expression, localization, and activity in the human retina and optic nerve head.Investigative ophthalmology & visual science · 2009Article
- The proprotein convertase PC5/6 is protective against intestinal tumorigenesis: in vivo mouse model.Molecular cancer · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several integrin alpha subunits undergo post-translational endoproteolytic processing at pairs of basic amino acids that is mediated by the proprotein convertase furin. Here we ask whether other convertase family members can participate in these processing events. We therefore examined the endoproteolysis rate of the integrin subunits pro-alpha5, alpha6 and alphav by recombinant furin, proprotein convertase (PC)5A, paired basic amino acid converting enzyme (PACE)4, PC1, PC2 and PC7 in vitro and/or ex vivo after overexpression in LoVo cells that were deficient in furin activity. We found that 60-fold more PC1 than furin was needed to produce 50% cleavage of pro-alpha subunit substrates in vitro; the defective pro-alpha chain endoproteolysis in LoVo cells was not rescued by overexpression of PC1 or PC2. No endoproteolysis occurred with PC7 either in vitro or ex vivo, although similar primary sequences of the cleavage site are found in integrins and in proteins efficiently processed by PC7, which suggests that a particular conformation of the cleavage site is required for optimal convertase-substrate interactions. In vitro, 50% cleavage of pro-alpha subunits was obtained with one-third of the amount of PC5A and PACE4 than of furin. In LoVo cells, PC5A remained more active than furin, PACE4 activity was quite low, and PC5B, which differs from PC5A by a C-terminal extension containing a transmembrane domain, was very inefficient in processing integrin alpha-subunit precursors. In conclusion, these results indicate that integrin alpha-subunit endoproteolytic processing involves the redundant function of furin and PC5A and to a smaller extent PACE4, but not of PC1, PC2, PC5B or PC7.
Indexed as
Identifiers
10657249PMC1220832W2096470048What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.