ArticleOncogene2000
Cooperative phosphorylation at multiple sites is required to activate p53 in response to UV radiation.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 88 citations in OpenAlex.
- Phosphorylation-dependent regulation of Eleven Lysine Rich Leukemia (ELL) uncovered by quantitative global phosphoproteomic analysis.Molecular genetics and genomics : MGG · 2026Article
- Ribosome biogenesis rate, a parameter of sensitivity to chemotherapeutic drugs inhibiting rRNA synthesis.Oncology reviews · 2025Review
- Maintaining Genome Integrity: Protein Kinases and Phosphatases Orchestrate the Balancing Act of DNA Double-Strand Breaks Repair in Cancer.International journal of molecular sciences · 2023Review
- Exogenous bacterial DnaK increases protein kinases activity in human cancer cell lines.Journal of translational medicine · 2021Article
- Cyclin B1/CDK1-regulated mitochondrial bioenergetics in cell cycle progression and tumor resistance.Cancer letters · 2019Review
- Novel role for p56/Lck in regulation of endothelial cell survival and angiogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Twist1 regulates keratinocyte proliferation and skin tumor promotion.Molecular carcinogenesis · 2016Article
- Article
- Cell cycle regulators guide mitochondrial activity in radiation-induced adaptive response.Antioxidants & redox signaling · 2014Review
- XPD-dependent activation of apoptosis in response to triplex-induced DNA damage.Nucleic acids research · 2013Article
- Prediction of functional phosphorylation sites by incorporating evolutionary information.Protein & cell · 2012Article
- A natural small molecule harmine inhibits angiogenesis and suppresses tumour growth through activation of p53 in endothelial cells.PloS one · 2012Article
- Curaxins: anticancer compounds that simultaneously suppress NF-κB and activate p53 by targeting FACT.Science translational medicine · 2011Article
- Phosphorylation network rewiring by gene duplication.Molecular systems biology · 2011Article
- Curcuminoids activate p38 MAP kinases and promote UVB-dependent signalling in keratinocytes.Experimental dermatology · 2010Article
- Reactivation of p53 in cells expressing hepatitis B virus X-protein involves p53 phosphorylation and a reduction of Hdm2.Cancer science · 2008Article
- Constitutive mTOR activation in TSC mutants sensitizes cells to energy starvation and genomic damage via p53.The EMBO journal · 2007Article
- Balance of Yin and Yang: ubiquitylation-mediated regulation of p53 and c-Myc.Neoplasia (New York, N.Y.) · 2006Review
- Defect in serine 46 phosphorylation of p53 contributes to acquisition of p53 resistance in oral squamous cell carcinoma cells.Oncogene · 2006Article
- Application of protein lysate microarrays to molecular marker verification and quantification.Proteome science · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The activity of the tumor suppressor p53 is induced in response to DNA-damaging agents such as UV and gamma radiation. Phosphorylation is one of the key regulatory steps for activating p53 function. Recent reports have shown that p53 is phosphorylated at both serines 15 and 392 in response to UV radiation. Phosphorylation at serine 15 prevents the binding of HDM2, a negative regulator of p53. Phosphorylation at serine 392 induces the DNA-binding function of p53. We examined the requirement for phosphorylation at both serines and show that both these modifications occur on the same molecule of p53. In vitro assays demonstrate that phosphorylation at either one of these sites is not sufficient to yield an active p53. Phosphorylation by DNA-PK, that modifies serines 15 and 37, inhibits HDM2 binding to p53 but does not induce the DNA-binding activity of p53. Phosphorylation at serine 392, on the other hand, stimulates the DNA-binding ability of p53 but does not make it immune to binding and inhibition by HDM2. Thus, our results demonstrate that multiple sites need to be modified to yield a functional p53.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.