ArticleJournal of clinical microbiology2000
Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
131 citing papers in PubMed, 404 citations in OpenAlex.
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- Antifungal Resistance and Synergistic Therapy in Candida Spp. Isolated From Bottlenose Dolphins Under Human Care: Implications for One Health Management.Veterinary medicine and science · 2025Article
- Colorectal cancer biofilm composition reveals distinct bacterial species signature.Applied microbiology and biotechnology · 2025Article
- Modeling reciprocal adaptation ofBiofilm · 2025Article
- Ribosome phenotypes for rapid classification of antibiotic-susceptible and resistant strains of Escherichia coli.Communications biology · 2025Article
- Fluoride-resistantFrontiers in microbiology · 2024Article
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- Differentiating methicillin resistant and susceptibleFrontiers in medicine · 2023Article
- Microbial Inoculants as Plant Biostimulants: A Review on Risk Status.Life (Basel, Switzerland) · 2022Review
- Rapid antibiotic susceptibility testing and species identification for mixed samples.Nature communications · 2022Article
- Assessment of genotypes, endosymbionts and clinical characteristics of Acanthamoeba recovered from ocular infection.BMC infectious diseases · 2022Article
- Colitis caused by Entamoeba histolytica identified by real-time-PCR and fluorescence in situ hybridization from formalin-fixed, paraffin-embedded tissue.European journal of microbiology & immunology · 2022Article
- Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection byAntibiotics (Basel, Switzerland) · 2022Article
- Fluorescence in situ hybridisation in Carnoy's fixed tonsil tissue.Scientific reports · 2022Article
- An outlook on fluorescent in situ hybridization coupled to flow cytometry as a versatile technique to evaluate the effects of foods and dietary interventions on gut microbiota.Archives of microbiology · 2022Review
- Fungi Can Be More Effective than Bacteria for the Bioremediation of Marine Sediments Highly Contaminated with Heavy Metals.Microorganisms · 2022Article
71 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using fluorescent in situ hybridization (FISH) with rRNA-targeted fluorescently labelled oligonucleotide probes, pathogens were rapidly detected and identified in positive blood culture bottles without cultivation and biotyping. In this study, 115 blood cultures with a positive growth index as determined by a continuous-reading automated blood culture system were examined by both conventional laboratory methods and FISH. For this purpose, oligonucleotide probes that allowed identification of approximately 95% of those pathogens typically associated with bacteremia were produced. The sensitivity and specificity of these probes were 100%. From all 115 blood cultures, microorganisms were grown after 1 day and identification to the family, genus, or species level was achieved after 1 to 3 days while 111 samples (96.5%) were similarly identified by FISH within 2.5 h. Staphylococci were identified in 62 of 62 samples, streptococci and enterococci were identified in 19 of 20 samples, gram-negative rods were identified in 28 of 30 samples, and fungi were identified in two of two samples. Thus, FISH is an appropriate method for identification of pathogens grown in blood cultures from septicemic patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.