ArticleJournal of clinical microbiology2000
Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 227 citations in OpenAlex.
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- Molecular characterization of enterotoxin genes in methicillin-resistant S. aureus isolated from food poisoning outbreaks in Egypt.Journal of health, population, and nutrition · 2023Article
- Metagenomics: The Next Culture-Independent Game Changer.Frontiers in microbiology · 2017Review
- The dormant blood microbiome in chronic, inflammatory diseases.FEMS microbiology reviews · 2015Review
- Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation.Frontiers in plant science · 2015Article
- Article
- Development of a broad-range 23S rDNA real-time PCR assay for the detection and quantification of pathogenic bacteria in human whole blood and plasma specimens.BioMed research international · 2013Article
- Evaluation of a PCR assay to detect enterococcusfaecalis in blood and determine glycopeptides resistance genes: van a and van B.Iranian journal of medical sciences · 2012Article
- Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting.Health research policy and systems · 2012Article
- Molecular detection, quantification, and diversity evaluation of microalgae.Marine biotechnology (New York, N.Y.) · 2012Review
- Bead array direct rRNA capture assay (rCapA) for amplification free speciation of Mycobacterium cultures.PloS one · 2012Article
- An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents.PloS one · 2011Article
- A microbial detection array (MDA) for viral and bacterial detection.BMC genomics · 2010Article
- Evaluation of one- and two-color gene expression arrays for microbial comparative genome hybridization analyses in routine applications.Journal of clinical microbiology · 2010Article
- Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides.BMC biotechnology · 2010Article
- Oligonucleotide microarray for the identification of potential mycotoxigenic fungi.BMC microbiology · 2010Article
- Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD).Journal of microbiological methods · 2010Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Current applications and future trends of molecular diagnostics in clinical bacteriology.Analytical and bioanalytical chemistry · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid identification of bacteria in blood cultures and other clinical specimens is important for patient management and antimicrobial therapy. We describe a rapid (<4 h) detection and identification system that uses universal PCR primers to amplify a variable region of bacterial 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides. This procedure was successful in discriminating a range of bacteria in pure cultures. When this procedure was applied directly to 158 unselected positive blood culture broths on the day when growth was detected, 125 (79.7%) were correctly identified, including 4 with mixed cultures. Nine (7.2%) yielded bacteria for which no oligonucleotide targets were present in the oligonucleotide panel, and 16 culture-positive broths (10.3%) produced no PCR product. In seven of the remaining eight broths, streptococci were identified but not subsequently grown, and one isolate of Staphylococcus aureus was misidentified as a coagulase-negative staphylococcus. The accuracy, range, and discriminatory power of the assay can be continually extended by adding further oligonucleotides to the panel without significantly increasing complexity or cost.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.