ArticleJournal of clinical microbiology2000
Differential diagnosis of Taenia saginata and Taenia solium infection by PCR.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Identification of risk areas and practices for Taenia saginata taeniosis/cysticercosis in Ethiopia: a systematic review and meta-analysis.Parasites & vectors · 2020Pooled it
- MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review.Parasites & vectors · 2019Pooled it
- The potential threat at the dining table:Parasite epidemiology and control · 2026Article
- Effect of Community Health Education Intervention on Prevalence and Pig Farmers' Knowledge, Attitudes, and Practices Related to Porcine Cysticercosis in Selected Areas of Tanzania.Veterinary medicine international · 2025Article
- Occurrence of Taenia species and Toxoplasma gondii in pigs slaughtered in Bujumbura city, Kayanza and Ngozi provinces, Burundi.BMC veterinary research · 2024Article
- Review
- Identification and phylogenetic analysis ofInternational journal for parasitology. Parasites and wildlife · 2023Article
- A rare case of human taeniasis caused byParasitology · 2023Article
- Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip.Frontiers in microbiology · 2023Article
- Evaluating Different Storage Media for Identification ofMicroorganisms · 2021Article
- Intestinal helminths as a biomolecular complex in archaeological research.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- HDP2: a ribosomal DNA (NTS-ETS) sequence as a target for species-specific molecular diagnosis of intestinal taeniasis in humans.Parasites & vectors · 2018Article
- Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Infection of Taenia asiatica in a Bai Person in Dali, China.The Korean journal of parasitology · 2016Article
- Current status of taeniasis and cysticercosis in Vietnam.The Korean journal of parasitology · 2014Review
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Laboratory diagnosis of Taenia asiatica in humans and animals.Tropical parasitology · 2013Article
- Histological and molecular biology diagnosis of neurocysticercosis in a patient without history of travel to endemic areas: case report.Parasite (Paris, France) · 2012Article
- Differentiating Taenia eggs found in human stools: does Ziehl-Neelsen staining help?Tropical medicine & international health : TM & IH · 2010Article
- Characterization of the Taenia spp HDP2 sequence and development of a novel PCR-based assay for discrimination of Taenia saginata from Taenia asiatica.Parasites & vectors · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia saginata and Taenia solium. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. saginata. The first, which is T. saginata specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1, 272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. saginata gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to both T. saginata and T. solium DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg.
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