ArticleJournal of clinical microbiology2000
Comparison of human papillomavirus detection and typing by cycle sequencing, line blotting, and hybrid capture.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 143 citations in OpenAlex.
- Inclusion of HPV testing in routine cervical cancer screening for women above 29 years in Germany: results for 8466 patients.British journal of cancer · 2003Trial
- Comparative evaluation of Allplex HPV28 and Anyplex II HPV28 assays for high-risk HPV genotyping in cervical samples.PloS one · 2025Article
- Human papillomavirus genotype concordance between Anyplex II HPV28 and linear array HPV genotyping test in anogenital samples.Journal of medical virology · 2022Article
- Evaluation of human papillomavirus (HPV) genotyping assays using type-specific HPV L1 reference DNA.Genes & genomics · 2021Article
- Article
- Hierarchical assessment of host factors influencing the spontaneous resolution of hepatitis C infection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- HPV Population Profiling in Healthy Men by Next-Generation Deep Sequencing Coupled with HPV-QUEST.Viruses · 2016Article
- Development of a fluorescence-based multiplex genotyping method for simultaneous determination of human papillomavirus infections and viral loads.BMC cancer · 2015Article
- From Human Papillomavirus (HPV) Detection to Cervical Cancer Prevention in Clinical Practice.Cancers · 2014Article
- Type-specific detection of 30 oncogenic human papillomaviruses by genotyping both E6 and L1 genes.Journal of clinical microbiology · 2013Article
- Predictors of human papillomavirus infection in women undergoing routine cervical cancer screening in Spain: the CLEOPATRE study.BMC infectious diseases · 2012Article
- Development of a bead-based multiplex genotyping method for diagnostic characterization of HPV infection.PloS one · 2012Article
- MassARRAY spectrometry is more sensitive than PreTect HPV-Proofer and consensus PCR for type-specific detection of high-risk oncogenic human papillomavirus genotypes in cervical cancer.Journal of clinical microbiology · 2011Article
- Detection of HPV by in situ hybridization in thin-layer (ThinPrep) cervicovaginal samples.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Human Papillomavirus in Brazilian women with and without cervical lesions.Virology journal · 2011Article
- Nucleic acid sequence-based amplification assay for human papillomavirus mRNA detection and typing: evidence for DNA amplification.Journal of clinical microbiology · 2010Article
- Signature sequence validation of human papillomavirus type 16 (HPV-16) in clinical specimens.Journal of clinical pathology · 2010Article
- Sensitive HPV detection in oropharyngeal cancers.BMC cancer · 2009Article
- Prevalence and type distribution of human papillomavirus in 5,000 British Columbia women--implications for vaccination.Cancer causes & control : CCC · 2009Article
- Validation of human papillomavirus genotyping by signature DNA sequence analysis.BMC clinical pathology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the results of human papillomavirus (HPV) detection and typing from 781 cervical samples assayed by three methods: L1 consensus PCR followed by cycle sequencing, L1 consensus PCR with biotinylated primers followed by hybridization to a line blot, and Hybrid Capture assay. Both PCR assays used L1 consensus PCR with primers MY09 and MY11. We evaluated the amplification efficiencies of both PCR assays and also compared the specific HPV types detected by each method. The samples positive by the Hybrid Capture assay were compared to the specific types detected by the PCR-based assays. The concordance between the two PCR assays in producing an HPV amplicon visible by gel electrophoresis or in detecting any HPV type was moderate: kappa values were 0.61 (95% confidence interval [CI] = 0.56 to 0.67) and 0.51 (95% CI = 0.46 to 0.58), respectively. The McNemar test for correlated proportions indicated that biotinylated PCR was less likely to produce a band (P = 0.001) and to detect an HPV type (P = 0.001) than the other PCR assay. In comparing the Hybrid Capture assay results with the HPV types detected by the PCR-based assays, we found that positivity by the Hybrid Capture assay for a number of samples may be due to cross-hybridization with HPV types not included in the Hybrid Capture assay probe cocktails.
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