ArticleJournal of clinical microbiology2000
Evaluation of the COBAS AMPLICOR CMV MONITOR test for detection of viral DNA in specimens taken from patients after liver transplantation.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- Cytomegalovirus pneumonia in a background of central nervous system tuberculosis.Respirology case reports · 2022Article
- A Practical Review of Cytomegalovirus in Gastroenterology and Hepatology.Gastroenterology research and practice · 2019Review
- An international multicenter performance analysis of cytomegalovirus load tests.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2013Article
- Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR.Medical microbiology and immunology · 2010Article
- Detection of cytomegalovirus (CMV) DNA in EDTA whole-blood samples: evaluation of the quantitative artus CMV LightCycler PCR kit in conjunction with automated sample preparation.Journal of clinical microbiology · 2008Article
- Evaluation of the ReSSQ assay in relation to the COBAS AMPLICOR CMV MONITOR test and an in-house nested PCR method for detection of cytomegalovirus DNA.Journal of clinical microbiology · 2005Article
- Internally controlled real-time PCR monitoring of adenovirus DNA load in serum or plasma of transplant recipients.Journal of clinical microbiology · 2005Article
- Evaluation of a new automated, standardized generic nucleic acid extraction method (total nucleic acid isolation kit) used in combination with cytomegalovirus DNA quantification by COBAS AMPLICOR CMV MONITOR.Journal of clinical microbiology · 2004Article
- Comparison of DNA amplification, mRNA amplification, and DNA hybridization techniques for detection of cytomegalovirus in bone marrow transplant recipients.Journal of clinical microbiology · 2003Article
- Comparison of molecular tests for detection and quantification of cell-associated cytomegalovirus DNA.Journal of clinical microbiology · 2003Article
- Comparison of LightCycler-based PCR, COBAS amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation.Journal of clinical microbiology · 2003Article
- Normalized quantification of human cytomegalovirus DNA by competitive real-time PCR on the LightCycler instrument.Journal of clinical microbiology · 2002Article
- Evaluation of the NucliSens CMV pp67 assay for detection and monitoring of human cytomegalovirus infection after allogeneic stem cell transplantation.Bone marrow transplantation · 2002Article
- Monitoring of viral load by quantitative plasma PCR during active cytomegalovirus infection of individual liver transplant patients.Journal of clinical microbiology · 2002Article
- Distinguishing cytomegalovirus (CMV) infection and disease with CMV nucleic acid assays.Journal of clinical microbiology · 2002Article
- Role of the laboratory in diagnosis and management of cytomegalovirus infection in hematopoietic stem cell and solid-organ transplant recipients.Journal of clinical microbiology · 2002Article
- Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems.Journal of clinical microbiology · 2001Article
- Evaluation of the Murex CMV DNA Hybrid Capture assay (version 2.0) for early diagnosis of cytomegalovirus infection in recipients of an allogeneic stem cell transplant.Bone marrow transplantation · 2001Article
- Comparison of three nucleic acid amplification assays of cerebrospinal fluid for diagnosis of cytomegalovirus encephalitis.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of cytomegalovirus (CMV) DNA in blood by PCR is a sensitive method for the detection of infection in patients posttransplantation. The test, however, has low specificity for the identification of overt CMV disease. Quantitative CMV PCR has been shown to overcome this shortcoming. The COBAS AMPLICOR CMV MONITOR test was evaluated by using consecutive serum and peripheral blood mononuclear cell (PBMN) samples from liver transplant patients. Twenty-five patients had CMV viremia (by shell vial cell culture assay) and/or tissue-invasive disease (by biopsy); 20 had no active infection. A total of 262 serum and 62 PBMN specimens were tested. Of 159 serum specimens from patients with overt CMV infection, the COBAS assay detected CMV DNA in 21 patients (sensitivity, 84%). Only 1 of 103 samples from patients with no evidence of active infection had detectable CMV DNA (341 copies/ml). By comparison of 62 matching serum and PBMN samples by the same assay, 12 PBMN samples were exclusively positive, whereas only 2 serum samples were exclusively positive (P < 0.05). At the time of clinical CMV infection, viral copy numbers were higher in PBMNs than serum from four of five patients. The COBAS AMPLICOR CMV MONITOR test is a sensitive and specific test for the quantitative detection of CMV DNA in blood. Clinical applications of the assay will require further validation with samples from a larger population of transplant patients.
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