ArticleJournal of clinical microbiology2000
Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 2 syntheses or guidelines pooled it, 289 citations in OpenAlex.
- Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.The Cochrane database of systematic reviews · 2019Pooled it
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Empirical voriconazole therapy for febrile neutropenic patients with hematological disorders: a prospective multicenter trial in Japan.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2013Trial
- Immediate versus deferred empirical antifungal (IDEA) therapy in high-risk patients with febrile neutropenia: a randomized, double-blind, placebo-controlled, multicenter study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Trial
- Madurella real-time PCR, a novel approach for eumycetoma diagnosis.PLoS neglected tropical diseases · 2020Article
- Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.Scientific reports · 2019Article
- Microfluidic Chip for Detection of Fungal Infections.ACS omega · 2019Article
- CX3CR1Science (New York, N.Y.) · 2018Article
- Comparison of Performance Characteristics of Aspergillus PCR in Testing a Range of Blood-Based Samples in Accordance with International Methodological Recommendations.Journal of clinical microbiology · 2016Article
- Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination.Journal of clinical microbiology · 2016Article
- Application of Culture-Independent Rapid Diagnostic Tests in the Management of Invasive Candidiasis and Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2015Review
- A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient.Current medical mycology · 2015Article
- Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis?BioMed research international · 2015Article
- Aspergillus galactomannan detection in comparison to a real-time PCR assay in serum samples from a high-risk group of patients.Central-European journal of immunology · 2015Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
- Succession patterns of fungi associated to wound-induced agarwood in wild Aquilaria malaccensis revealed from quantitative PCR assay.World journal of microbiology & biotechnology · 2014Article
- Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients.Journal of clinical microbiology · 2013Article
- Detection of invasive aspergillosis in bone marrow transplant recipients using real-time PCR.Journal of global infectious diseases · 2013Article
- Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome.Scientifica · 2013Review
- Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.Journal of clinical microbiology · 2012Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The sensitivity of the assay was comparable to those of previously described PCR protocols (5 CFU/ml). Specific detection of C. albicans and A. fumigatus could be achieved. The assay showed a high reproducibility of 96 to 99%. The assay was linear in a range between 10(1) and 10(4) Aspergillus conidia. As capillaries do not have to be reopened for post-PCR analysis, the risk of carryover contaminations could be minimized. The Light Cycler allowed quantification of the fungal loads in a limited number of clinical specimens from patients with hematological malignancies and histologically proven invasive fungal infections. Five of nine positive samples had fungal loads between 5 and 10 CFU/ml of blood, two of nine positive samples had fungal loads between 10 and 100 CFU/ml of blood, and two of nine samples had fungal loads of more than 100 CFU/ml of blood. All samples were also found to be PCR positive by PCR-enzyme-linked immunosorbent assay analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.