ArticleJournal of clinical microbiology2000
Simplified hepatitis C virus genotyping by heteroduplex mobility analysis.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 86 citations in OpenAlex.
- Injecting drug use and hepatitis C virus infection independently increase biomarkers of inflammatory disease risk which are incompletely restored by curative direct-acting antiviral therapy.Frontiers in immunology · 2024Article
- Envelope-Specific IgG3 and IgG1 Responses Are Associated with Clearance of Acute Hepatitis C Virus Infection.Viruses · 2020Article
- HCV Detection, Discrimination, and Genotyping Technologies.Sensors (Basel, Switzerland) · 2018Review
- Performance of 6 HCV genotyping 9G test for HCV genotyping in clinical samples.Virology journal · 2018Article
- Genotyping & diagnostic methods for hepatitis C virus: A need of low-resource countries.The Indian journal of medical research · 2018Review
- Article
- Incident Hepatitis C Virus Genotype Distribution and Multiple Infection in Australian Prisons.Journal of clinical microbiology · 2016Article
- A method for near full-length amplification and sequencing for six hepatitis C virus genotypes.BMC genomics · 2016Article
- Cross-genotypic examination of hepatitis C virus polymerase inhibitors reveals a novel mechanism of action for thumb binders.Antimicrobial agents and chemotherapy · 2014Article
- Hepatitis C virus genotype 6: virology, epidemiology, genetic variation and clinical implication.World journal of gastroenterology · 2014Review
- Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases.Genetics and molecular biology · 2014Article
- Inferring viral population structures using heteroduplex mobility and DNA sequence analyses.Journal of virological methods · 2013Article
- Development and Application of an In-house Line Probe Assay for Hepatitis C Virus Genotyping.Hepatitis monthly · 2013Article
- Introduction of an automated system for the diagnosis and quantification of hepatitis B and hepatitis C viruses.The open virology journal · 2012Article
- Correlation of the 5'untranslated region (5'UTR) and non-structural 5B (NS5B) nucleotide sequences in hepatitis C virus subtyping.International journal of molecular epidemiology and genetics · 2010Article
- High-affinity aptamers to subtype 3a hepatitis C virus polymerase display genotypic specificity.Antimicrobial agents and chemotherapy · 2006Article
- Multitypic hepatitis C virus infection identified by real-time nucleotide sequencing of minority genotypes.Journal of clinical microbiology · 2006Article
- Analysis of the 5' noncoding region versus the NS5b region in genotyping hepatitis C virus isolates from blood donors in France.Journal of clinical microbiology · 2006Article
- Development of multiplex PCRs for detection of common viral pathogens and agents of congenital infections.Journal of clinical microbiology · 2005Article
- Transmission of human hepatitis C virus from patients in secondary cells for long term culture.Virology journal · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heteroduplex mobility analysis (HMA) was used to genotype hepatitis C viruses (HCV) with PCR fragments derived from the 5' untranslated region (5'-UTR) or the NS5b region. HCV 5'-UTR fragments were amplified from 296 serum samples by use of a combined reverse transcription-PCR assay, and the genotypes of isolates were determined by sequencing. HCV genotype distributions in Australia were 39% for genotype 1a, 15% for 1b, 3% for 1a/b, <1% for 2a/c, 5% for 2b, 34% for 3a, <1% for 3b, and 1% for 4, and 1% of patients were infected with more than one genotype. Pairwise HMA of subtypes 1a, 1b, 2a/c, 2b, 3a, 3b, 4a, and 6a demonstrated that five distinct heteroduplex patterns were formed between the eight subtypes. A reference panel that contained a representative of each pattern (1a, 2b, 3a, 4a, and 6a) was used for genotyping. The pattern of heteroduplexes formed when a test isolate was mixed with the five reference isolates was correlated with the genotype, as determined by sequencing. Genotypes determined by HMA correlated exactly with sequencing results within the groups 1, 2, 3a, 3b/4, and 6. HMA was also used to simplify the identification of mixed infection with two HCV genotypes. In further studies, with amplicons from the NS5b region, HMA classified isolates into their respective subtypes, and the heteroduplex mobility ratio correlated closely with nucleotide sequence variation at the isolate, subtype, and genotype levels. HMA provides an adaptable, inexpensive, and rapid method of genotyping HCV that requires fewer resources than DNA sequencing.
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