ArticleGenetics2000
Functions of fission yeast orp2 in DNA replication and checkpoint control.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Targeted Forward Genetics: Population-Scale Analyses of Allele Replacements Spanning Thousands of Base Pairs in Fission Yeast.G3 (Bethesda, Md.) · 2019Article
- Article
- Sequential and counter-selectable cassettes for fission yeast.BMC biotechnology · 2016Article
- Rapid, efficient and precise allele replacement in the fission yeast Schizosaccharomyces pombe.Current genetics · 2014Article
- Article
- Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.Molecular genetics and genomics : MGG · 2009Article
- Low-copy episomal vector pFY20 and high-saturation coverage genomic libraries for the fission yeast Schizosaccharomyces pombe.Yeast (Chichester, England) · 2008Article
- Antagonism of Chk1 signaling in the G2 DNA damage checkpoint by dominant alleles of Cdr1.Genetics · 2006Article
- Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.Journal of cell science · 2006Article
- A microbial TRP-like polycystic-kidney-disease-related ion channel gene.The Biochemical journal · 2005Article
- Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance.The EMBO journal · 2004Article
- In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.Methods (San Diego, Calif.) · 2004Article
- Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR.Nucleic acids research · 2003Article
- A conserved domain of Schizosaccharomyces pombe dfp1(+) is uniquely required for chromosome stability following alkylation damage during S phase.Molecular and cellular biology · 2002Article
- Control of DNA rereplication via Cdc2 phosphorylation sites in the origin recognition complex.Molecular and cellular biology · 2001Article
- Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomycespombe.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
orp2 is an essential gene of the fission yeast Schizosaccharomyces pombe with 22% identity to budding yeast ORC2. We isolated temperature-sensitive alleles of orp2 using a novel plasmid shuffle based on selection against thymidine kinase. Cells bearing the temperature-sensitive allele orp2-2 fail to complete DNA replication at a restrictive temperature and undergo cell cycle arrest. Cell cycle arrest depends on the checkpoint genes rad1 and rad3. Even when checkpoint functions are wild type, the orp2-2 mutation causes high rates of chromosome and plasmid loss. These phenotypes support the idea that Orp2 is a replication initiation factor. Selective spore germination allowed analysis of orp2 deletion mutants. These experiments showed that in the absence of orp2 function, cells proceed into mitosis despite a lack of DNA replication. This suggests either that the Orp2 protein is a part of the checkpoint machinery or more likely that DNA replication initiation is required to induce the replication checkpoint signal.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.