ArticleBiophysical journal2000
Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 83 citations in OpenAlex.
- Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography.Nature communications · 2025Article
- Lipid Dynamics in Diisobutylene-Maleic Acid (DIBMA) Lipid Particles in Presence of Sensory Rhodopsin II.International journal of molecular sciences · 2021Article
- Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.Annual review of biochemistry · 2017Review
- Mechanism divergence in microbial rhodopsins.Biochimica et biophysica acta · 2014Review
- Photolithographic fabrication of microapertures with well-defined, three-dimensional geometries for suspended lipid membrane studies.Analytical chemistry · 2013Article
- A dry membrane protection technique to allow surface acoustic wave biosensor measurements of biological model membrane approaches.Sensors (Basel, Switzerland) · 2013Article
- Tuning the primary reaction of channelrhodopsin-2 by imidazole, pH, and site-specific mutations.Biophysical journal · 2012Article
- The signal transfer from the receptor NpSRII to the transducer NpHtrII is not hampered by the D75N mutation.Biophysical journal · 2011Article
- The photochemical reaction cycle and photoinduced proton transfer of sensory rhodopsin II (Phoborhodopsin) from Halobacterium salinarum.Biophysical journal · 2010Article
- Azide as a probe of proton transfer reactions in photosynthetic oxygen evolution.Biophysical journal · 2008Article
- Laser-induced transient grating analysis of dynamics of interaction between sensory rhodopsin II D75N and the HtrII transducer.Biophysical journal · 2007Article
- Correlation of the O-intermediate rate with the pKa of Asp-75 in the dark, the counterion of the Schiff base of Pharaonis phoborhodopsin (sensory rhodopsin II).Biophysical journal · 2005Article
- Time-resolved detection of sensory rhodopsin II-transducer interaction.Biophysical journal · 2004Article
- Role of Arg-72 of pharaonis Phoborhodopsin (sensory rhodopsin II) on its photochemistry.Biophysical journal · 2004Article
- Complete sequence and molecular characterization of pNB101, a rolling-circle replicating plasmid from the haloalkaliphilic archaeon Natronobacterium sp. strain AS7091.Extremophiles : life under extreme conditions · 2004Article
- Time-resolved FTIR studies of sensory rhodopsin II (NpSRII) from Natronobacterium pharaonis: implications for proton transport and receptor activation.Biophysical journal · 2003Article
- FTIR spectroscopy of the M photointermediate in pharaonis rhoborhodopsin.Biophysical journal · 2002Article
- Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).Biophysical journal · 2002Article
- Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.Biophysical journal · 2002Article
- Probing the proton channel and the retinal binding site of Natronobacterium pharaonis sensory rhodopsin II.Biophysical journal · 2002Article
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present work the light-activated proton transfer reactions of sensory rhodopsin II from Natronobacterium pharaonis (pSRII) and those of the channel-mutants D75N-pSRII and F86D-pSRII are investigated using flash photolysis and black lipid membrane (BLM) techniques. Whereas the photocycle of the F86D-pSRII mutant is quite similar to that of the wild-type protein, the photocycle of D75N-pSRII consists of only two intermediates. The addition of external proton donors such as azide, or in the case of F86D-pSRII, imidazole, accelerates the reprotonation of the Schiff base, but not the turnover. The electrical measurements prove that pSRII and F86D-pSRII can function as outwardly directed proton pumps, whereas the mutation in the extracellular channel (D75N-pSRII) leads to an inwardly directed transient current. The almost negligible size of the photostationary current is explained by the long-lasting photocycle of about a second. Although the M decay, but not the photocycle turnover, of pSRII and F86D-pSRII is accelerated by the addition of azide, the photostationary current is considerably increased. It is discussed that in a two-photon process a late intermediate (N- and/or O-like species) is photoconverted back to the original resting state; thereby the long photocycle is cut short, giving rise to the large increase of the photostationary current. The results presented in this work indicate that the function to generate ion gradients across membranes is a general property of archaeal rhodopsins.
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