ArticleBiophysical journal2000
Direct inhibition of microtubule-based kinesin motility by local anesthetics.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 59 citations in OpenAlex.
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain.bioRxiv : the preprint server for biology · 2026Article
- Kinesin-1 and mitochondrial motility control by discrimination of structurally equivalent but distinct subdomains in Ran-GTP-binding domains of Ran-binding protein 2.Open biology · 2013Article
- Local anesthetics inhibit kinesin motility and microtentacle protrusions in human epithelial and breast tumor cells.Breast cancer research and treatment · 2011Article
- Dynamics of native β-actin mRNA transport in the cytoplasm.Traffic (Copenhagen, Denmark) · 2011Article
- An oviposition stimulant binding protein in a butterfly: Immunohistochemical localization and electrophysiological responses to plant compounds.Communicative & integrative biology · 2009Article
- Pressure-induced changes in the structure and function of the kinesin-microtubule complex.Biophysical journal · 2009Article
- Long-range cooperative binding of kinesin to a microtubule in the presence of ATP.The Journal of cell biology · 2005Article
- Motility of single one-headed kinesin molecules along microtubules.Biophysical journal · 2001Article
- Reconstitution of brefeldin A-induced golgi tubulation and fusion with the endoplasmic reticulum in semi-intact chinese hamster ovary cells.Molecular biology of the cell · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Local anesthetics are known to inhibit neuronal fast anterograde axoplasmic transport (FAAT) in a reversible and dose-dependent manner, but the precise mechanism has not been determined. FAAT is powered by kinesin superfamily proteins, which transport membranous organelles, vesicles, or protein complexes along microtubules. We investigated the direct effect of local anesthetics on kinesin, using both in vitro motility and single-molecule motility assays. In the modified in vitro motility assay, local anesthetics immediately and reversibly stopped the kinesin-based microtubule movement in an all-or-none fashion without lowering kinesin ATPase activity. QX-314, a permanently charged derivative of lidocaine, exerted an effect similar to that of lidocaine, suggesting that the effect of anesthetics is due to the charged form of the anesthetics. In the single-molecule motility assay, the local anesthetic tetracaine inhibited the motility of individual kinesin molecules in a dose-dependent manner. The concentrations of the anesthetics that inhibited the motility of kinesin correlated well with those blocking FAAT. We conclude that the charged form of local anesthetics directly and reversibly inhibits kinesin motility in a dose-dependent manner, and it is the major cause of the inhibition of FAAT by local anesthetics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.