ArticleApplied and environmental microbiology2000
Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 93 citations in OpenAlex.
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- Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile ofFrontiers in microbiology · 2021Article
- Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies inFrontiers in bioengineering and biotechnology · 2021Review
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
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- Genetics, Molecular, and Proteomics Advances in Filamentous Fungi.Current microbiology · 2017Review
- The Protein Disulfide Isomerase ofFrontiers in microbiology · 2017Article
- Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.Microbial biotechnology · 2016Article
- Transcriptomic insights into the physiology of Aspergillus niger approaching a specific growth rate of zero.Applied and environmental microbiology · 2010Article
- HacA-dependent transcriptional switch releases hacA mRNA from a translational block upon endoplasmic reticulum stress.Eukaryotic cell · 2009Article
- Transcriptomic comparison of Aspergillus niger growing on two different sugars reveals coordinated regulation of the secretory pathway.BMC genomics · 2009Article
- Cloning of the cytochrome p450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomyces dendrorhous.BMC microbiology · 2008Article
- Genomic analysis of the secretion stress response in the enzyme-producing cell factory Aspergillus niger.BMC genomics · 2007Article
- The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora.Molecular microbiology · 2007Article
- Generation, annotation, and analysis of an extensive Aspergillus niger EST collection.BMC microbiology · 2006Article
- HacA-independent induction of chaperone-encoding gene bipA in Aspergillus niger strains overproducing membrane proteins.Applied and environmental microbiology · 2006Article
- UPR-independent dithiothreitol stress-induced genes in Aspergillus niger.Molecular genetics and genomics : MGG · 2005Article
- Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30.Applied and environmental microbiology · 2005Article
- Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.Applied and environmental microbiology · 2004Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding of denatured and reduced RNase A. pdiA also complemented PDI function in a Saccharomyces cerevisiae Deltapdi1 mutant in a yeast-based killer toxin assay. Levels of pdiA mRNA and PDIA protein were raised by the accumulation of unfolded proteins in the endoplasmic reticulum. This response of pdiA mRNA levels was slower and lower in magnitude than that of A. niger bipA, suggesting that the induction of pdiA is not part of the primary stress response. An increased level of pdiA transcripts was also observed in two A. niger strains overproducing a heterologous protein, hen egg white lysozyme (HEWL). Although overexpression of PDI has been successful in increasing yields of some heterologous proteins in S. cerevisiae, overexpression of PDIA did not increase secreted yields of HEWL in A. niger, suggesting that PDIA itself is not limiting for secretion of this protein. Downregulation of pdiA by antisense mRNA reduced the levels of microsomal PDIA activity by up to 50%, lowered the level of PDIA as judged by Western blots, and lowered the secreted levels of glucoamylase by 60 to 70%.
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