ArticleApplied and environmental microbiology2000
Expression of the Staphylococcus hyicus lipase in Lactococcus lactis.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 53 citations in OpenAlex.
- Roles of Mature Domain Targeting Signals (MTSs) for Protein Translocation and Secretion inInternational journal of molecular sciences · 2024Article
- Shifting the pH profiles of Staphylococcus epidermidis lipase (SEL) and Staphylococcus hyicus lipase (SHL) through generating chimeric lipases by DNA shuffling strategy.World journal of microbiology & biotechnology · 2024Article
- A Review of the Microbial Production of Bioactive Natural Products and Biologics.Frontiers in microbiology · 2019Review
- Factors Affecting Inducible Expression of Outer Membrane Protein A (OmpA) of Shigella dysenteriae Type-1 in Lactococcus lactis Using Nisin Inducible Controlled Expression (NICE).Indian journal of microbiology · 2016Article
- Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis.Microbial cell factories · 2012Article
- Enzyme replacement therapy for pancreatic insufficiency: present and future.Clinical and experimental gastroenterology · 2011Article
- Expression of an organic solvent stable lipase from Staphylococcus epidermidis AT2.International journal of molecular sciences · 2010Article
- Gene cloning, expression, and characterization of the Geobacillus Thermoleovorans CCR11 thermoalkaliphilic lipase.Molecular biotechnology · 2009Article
- Protein secretion in Lactococcus lactis : an efficient way to increase the overall heterologous protein production.Microbial cell factories · 2005Article
- The peptidyl-prolyl isomerase motif is lacking in PmpA, the PrsA-like protein involved in the secretion machinery of Lactococcus lactis.Applied and environmental microbiology · 2002Article
- Controlled production of stable heterologous proteins in Lactococcus lactis.Applied and environmental microbiology · 2002Article
- Oral treatment with Lactococcus lactis expressing Staphylococcus hyicus lipase enhances lipid digestion in pigs with induced pancreatic insufficiency.Applied and environmental microbiology · 2002Article
- Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extracellular Staphylococcus hyicus lipase was expressed under the control of different promoters in Lactococcus lactis and Bacillus subtilis. Its expression at high and moderate levels is toxic for the former and the latter hosts, respectively. In L. lactis, the lipase was expressed at a high level, up to 30% of the total cellular proteins, under the control of the inducible promoter PnisA. About 80% of the lipase remained associated with the cells. Close to half of this amount remained associated with the inner side of the cytoplasmic membrane as unprocessed pre-pro-lipase. The other half was trapped by the cell wall and partially degraded at the N-terminal end. This result suggests that extracellular proteases degrade the lipase. Surprisingly, the kinetics and the pattern of lipase degradation were different in the two L. lactis subspecies, L. lactis subsp. cremoris and L. lactis subsp. lactis. The extracellular proteolytic systems that degrade lipase are thus different in these closely related subspecies. The incorrect export of the lipase is not due to an inappropriate leader peptide but may be due to an inefficiency of several steps of lipase secretion. We propose that (i) the S. hyicus lipase may require a special accessory system to be correctly exported or (ii) the kinetics of lipase synthesis may be a critical factor for proper folding.
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