ArticleNucleic acids research2000
RNA double cleavage by a hairpin-derived twin ribozyme.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- A programmable ribozyme for RNA signal transduction.Nature communications · 2026Article
- Multivalent DNAzyme agents for cleaving folded RNA.Nucleic acids research · 2024Article
- Synthesis of specifically modified oligonucleotides for application in structural and functional analysis of RNA.Journal of nucleic acids · 2011Article
- Ligand-induced conformational capture of a synthetic tetracycline riboswitch revealed by pulse EPR.RNA (New York, N.Y.) · 2011Article
- Free energy estimation of short DNA duplex hybridizations.BMC bioinformatics · 2010Article
- A triplex ribozyme expression system based on a single hairpin ribozyme.Oligonucleotides · 2008Article
- Free energy landscapes of RNA/RNA complexes: with applications to snRNA complexes in spliceosomes.Journal of molecular biology · 2006Article
- The role of phosphate groups in the VS ribozyme-substrate interaction.Nucleic acids research · 2004Article
- Selenium derivatization and crystallization of DNA and RNA oligonucleotides for X-ray crystallography using multiple anomalous dispersion.Nucleic acids research · 2004Article
- A continuous kinetic assay for RNA-cleaving deoxyribozymes, exploiting ethidium bromide as an extrinsic fluorescent probe.Nucleic acids research · 2002Article
- Ribozyme suppression of endogenous thyroid hormone receptor activity in Xenopus laevis cells.Nucleic acids research · 2002Article
- A peptide template as an allosteric supramolecular catalyst for the cleavage of phosphate esters.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hairpin ribozyme is a small catalytic RNA that catalyses reversible sequence-specific RNA hydrolysis in trans. It consists of two domains, which interact with each other by docking in an antiparallel fashion. There is a region between the two domains acting as a flexible hinge for interdomain interactions to occur. Hairpin ribozymes with reverse-joined domains have been constructed by dissecting the domains at the hinge and rejoining them in reverse order. We have used both the conventional and reverse-joined hairpin ribozymes for the design of a hairpin-derived twin ribozyme. We show that this twin ribozyme cleaves a suitable RNA substrate at two specific sites while maintaining the target specificity of the individual monoribozymes. For characterisation of the studied ribozymes we have evaluated a quantitative assay of sequence-specific ribozyme activity using fluorescently labelled RNA substrates in conjunction with an automated DNA sequencer. This assay was found to be applicable with hairpin and hairpin-derived ribozymes. The results demonstrate the potential of hairpin ribozymes for multi-target strategies of RNA cleavage and suggest the possibility for employing hairpin-derived twin ribozymes as powerful tools for RNA manipulation in vitro and in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.