ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
The presynaptic calcium channel is part of a transmembrane complex linking a synaptic laminin (alpha4beta2gamma1) with non-erythroid spectrin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 65 citations in OpenAlex.
- Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction.Frontiers in synaptic neuroscience · 2021Article
- The role of laminins in the organization and function of neuromuscular junctions.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Loss of β2-laminin alters calcium sensitivity and voltage-gated calcium channel maturation of neurotransmission at the neuromuscular junction.The Journal of physiology · 2015Article
- Spectrin Breakdown Products (SBDPs) as Potential Biomarkers for Neurodegenerative Diseases.Current translational geriatrics and experimental gerontology reports · 2012Article
- Regulation of synaptic vesicle docking by different classes of macromolecules in active zone material.PloS one · 2012Article
- Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of α-dystrobrevin.The Journal of cell biology · 2011Article
- Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.Journal of neurochemistry · 2010Article
- Membrane domains based on ankyrin and spectrin associated with cell-cell interactions.Cold Spring Harbor perspectives in biology · 2009Review
- Mutations in LAMB2 causing a severe form of synaptic congenital myasthenic syndrome.Journal of medical genetics · 2009Article
- The Na-K-ATPase and calcium-signaling microdomains.Physiology (Bethesda, Md.) · 2008Review
- Neurite outgrowth and in vivo sensory innervation mediated by a Ca(V)2.2-laminin beta 2 stop signal.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Imaging synaptosomal calcium concentration microdomains and vesicle fusion by using total internal reflection fluorescent microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions.The Journal of investigative dermatology · 2006Article
- Beta1 integrins in muscle, but not in motor neurons, are required for skeletal muscle innervation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- The tissue plasminogen activator-plasmin system participates in the rewarding effect of morphine by regulating dopamine release.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Altered properties of quantal neurotransmitter release at endplates of mice lacking P/Q-type Ca2+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Direct measurement of single-channel Ca(2+) currents in bullfrog hair cells reveals two distinct channel subtypes.The Journal of physiology · 2001Article
- Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Active zones on motor nerve terminals contain alpha 3beta 1 integrin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Nerve regeneration studies at the neuromuscular junction (NMJ) suggest that synaptic basal lamina components tell the returning axon where to locate neurotransmitter release machinery, including synaptic vesicle clusters and active zones. Good candidates for these components are the synaptic laminins (LNs) containing alpha4, alpha5, or beta2 chains. Results from a beta2 laminin knockout mouse have suggested a linkage of this extracellular laminin to cytosolic synaptic vesicle clusters. Here we report such a transmembrane link at the electric organ synapse, which is homologous to the NMJ. We immunopurified electric organ synaptosomes and found on their surface two laminins of 740 and 900 kDa. The 740 kDa laminin has a composition of alpha4beta2gamma1 (laminin-9). Immunostaining reveals that as in the NMJ, alpha4 and beta2 chains are concentrated at the electric organ synapse. Using detergent-solubilized synaptosomes, we immunoprecipitated a complex containing alpha4beta2gamma1 laminin, the voltage-gated calcium channel, and the cytoskeletal protein spectrin. Other presynaptic proteins such as 900 kDa laminin are not found in this complex. We hypothesize that alpha4beta2gamma1 laminin in the synaptic basal lamina attaches to calcium channel, which in turn is attached to cytosolic spectrin. Spectrin could then organize synaptic vesicle clusters by binding vesicle-associated proteins.
Indexed as
Identifiers
10648706PMC6774149W1487299688What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.