ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Relief of G-protein inhibition of calcium channels and short-term synaptic facilitation in cultured hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 105 citations in OpenAlex.
- A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.Nature methods · 2024Article
- A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits.bioRxiv : the preprint server for biology · 2023Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
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- Autaptic Cultures: Methods and Applications.Frontiers in synaptic neuroscience · 2020Article
- Retinoid receptor-based signaling plays a role in voltage-dependent inhibition of invertebrate voltage-gated CaThe Journal of biological chemistry · 2019Article
- Presynaptic Calcium Channels.International journal of molecular sciences · 2019Review
- PKC enhances the capacity for secretion by rapidly recruiting covert voltage-gated Ca2+ channels to the membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Distinct roles for Cav2.1-2.3 in activity-dependent synaptic dynamics.Journal of neurophysiology · 2014Article
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- Regulation of Ca(V)2 calcium channels by G protein coupled receptors.Biochimica et biophysica acta · 2013Review
- GABAB receptor-mediated frequency-dependent and circadian changes in synaptic plasticity modulate retinal input to the suprachiasmatic nucleus.The Journal of physiology · 2013Article
- Epileptiform activity in the CA1 region of the hippocampus becomes refractory to attenuation by cannabinoids in part because of endogenous γ-aminobutyric acid type B receptor activity.Journal of neuroscience research · 2012Article
- Signaling complexes of voltage-gated sodium and calcium channels.Neuroscience letters · 2010Review
- Short-term potentiation of mEPSCs requires N-, P/Q- and L-type Ca2+ channels and mitochondria in the supraoptic nucleus.The Journal of physiology · 2008Article
- Alternative splicing controls G protein-dependent inhibition of N-type calcium channels in nociceptors.Nature neuroscience · 2007Article
- Functional consequences of presynaptic inhibition during behaviorally relevant activity.Journal of neurophysiology · 2006Article
- Modeling Starburst cells' GABA(B) receptors and their putative role in motion sensitivity.Biophysical journal · 2006Article
- Presynaptic, activity-dependent modulation of cannabinoid type 1 receptor-mediated inhibition of GABA release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Presynaptic N-type and P/Q-type Ca2+ channels mediating synaptic transmission at the calyx of Held of mice.The Journal of physiology · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-protein inhibition of voltage-gated calcium channels can be transiently relieved by repetitive physiological stimuli. Here, we provide evidence that such relief of inhibition contributes to short-term synaptic plasticity in microisland-cultured hippocampal neurons. With G-protein inhibition induced by the GABA(B) receptor agonist baclofen or the adenosine A1 receptor agonist 2-chloroadenosine, short-term synaptic facilitation emerged during action potential trains. The facilitation decayed with a time constant of approximately 100 msec. However, addition of the calcium channel inhibitor Cd(2+) at 2-3 microM had no such effect and did not alter baseline synaptic depression. As expected of facilitation from relief of channel inhibition, analysis of miniature EPSCs implicated presynaptic modulation, and elevating presynaptic Ca(2+) entry blunted the facilitation. Most telling was the near occlusion of synaptic facilitation after selective blockade of P/Q- but not N-type calcium channels. This was as predicted from experiments using recombinant calcium channels expressed in human embryonic kidney (HEK) 293 cells; we found significantly stronger relief of G-protein inhibition in recombinant P/Q- versus N-type channels during action potential trains. G-protein inhibition in HEK 293 cells was induced via recombinant M2 muscarinic acetylcholine receptors activated by carbachol, an acetylcholine analog. Thus, relief of G-protein inhibition appears to produce a novel form of short-term synaptic facilitation in cultured neurons. Similar short-term synaptic plasticity may be present at a wide variety of synapses, as it could occur during autoreceptor inhibition by glutamate or GABA, heterosynaptic inhibition by GABA, tonic adenosine inhibition, and in many other instances.
Indexed as
Identifiers
10648693PMC6774171W2123988448What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.