ArticleMolecular and cellular biology2000
Yeast meiosis-specific protein Hop1 binds to G4 DNA and promotes its formation.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- The ATPase activity of yeast chromosome axis protein Hop1 affects the frequency of meiotic crossovers.Nucleic acids research · 2025Article
- NMR Screen Reveals the Diverse Structural Landscape of a G-Quadruplex Library.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Article
- Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae.Nucleic acids research · 2022Article
- How do small chromosomes know they are small? Maximizing meiotic break formation on the shortest yeast chromosomes.Current genetics · 2021Review
- Folding and persistence times of intramolecular G-quadruplexes transiently embedded in a DNA duplex.Nucleic acids research · 2021Article
- G-quadruplexes may determine the landscape of recombination in HSV-1.BMC genomics · 2019Article
- Extreme clustering of type-1 NF1 deletion breakpoints co-locating with G-quadruplex forming sequences.Human genetics · 2018Article
- Mms1 is an assistant for regulating G-quadruplex DNA structures.Current genetics · 2018Review
- Article
- Probing the Potential Role of Non-B DNA Structures at Yeast Meiosis-Specific DNA Double-Strand Breaks.Biophysical journal · 2017Article
- Amoebozoans Are Secretly but Ancestrally Sexual: Evidence for Sex Genes and Potential Novel Crossover Pathways in Diverse Groups of Amoebae.Genome biology and evolution · 2017Article
- Topoisomerase IB of Deinococcus radiodurans resolves guanine quadruplex DNA structures in vitro.Journal of biosciences · 2015Article
- In-silico modeling studies of G-quadruplex with soy isoflavones having anticancerous activity.Journal of molecular modeling · 2015Article
- Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- HIV-1 nucleocapsid proteins as molecular chaperones for tetramolecular antiparallel G-quadruplex formation.Journal of the American Chemical Society · 2013Article
- Article
- DNA secondary structures: stability and function of G-quadruplex structures.Nature reviews. Genetics · 2012Review
- G-quadruplexes-novel mediators of gene function.Journal of cardiovascular translational research · 2011Review
- RTEL1: an essential helicase for telomere maintenance and the regulation of homologous recombination.Nucleic acids research · 2011Review
- G-quadruplex DNA sequences are evolutionarily conserved and associated with distinct genomic features in Saccharomyces cerevisiae.PLoS computational biology · 2010Article
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3 authors.
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Abstract
DNA molecules containing stretches of contiguous guanine residues can assume a stable configuration in which planar quartets of guanine residues joined by Hoogsteen pairing appear in a stacked array. This conformation, called G4 DNA, has been implicated in several aspects of chromosome behavior including immunoglobulin gene rearrangements, promoter activation, and telomere maintenance. Moreover, the ability of the yeast SEP1 gene product to cleave DNA in a G4-DNA-dependent fashion, as well as that of the SGS1 gene product to unwind G4 DNA, has suggested a crucial role for this structure in meiotic synapsis and recombination. Here, we demonstrate that the HOP1 gene product, which plays a crucial role in the formation of synaptonemal complex in Saccharomyces cerevisiae, binds robustly to G4 DNA. The apparent dissociation constant for interaction with G4 DNA is 2 x 10(-10), indicative of binding that is about 1,000-fold stronger than to normal duplex DNA. Oligonucleotides of appropriate sequence bound Hop1 protein maximally if the DNA was first subjected to conditions favoring the formation of G4 DNA. Furthermore, incubation of unfolded oligonucleotides with Hop1 led to their transformation into G4 DNA. Methylation interference experiments confirmed that modifications blocking G4 DNA formation inhibit Hop1 binding. In contrast, neither bacterial RecA proteins that preferentially interact with GT-rich DNA nor histone H1 bound strongly to G4 DNA or induced its formation. These findings implicate specific interactions of Hop1 protein with G4 DNA in the pathway to chromosomal synapsis and recombination in meiosis.
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