ArticleMolecular and cellular biology2000
TATA-Binding protein-TATA interaction is a key determinant of differential transcription of silkworm constitutive and silk gland-specific tRNA(Ala) genes.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
- Insights from a chum salmon (Oncorhynchus keta) genome assembly regarding whole-genome duplication and nucleotide variation influencing gene function.G3 (Bethesda, Md.) · 2023Article
- TATA-Like Boxes in RNA Polymerase III Promoters: Requirements for Nucleotide Sequences.International journal of molecular sciences · 2020Article
- Differential expression of human tRNA genes drives the abundance of tRNA-derived fragments.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular organization of the 5S rDNA gene type II in elasmobranchs.RNA biology · 2016Article
- Stabilization of eukaryotic ribosomal termination complexes by deacylated tRNA.Nucleic acids research · 2015Article
- Emerging roles of tRNA in adaptive translation, signalling dynamics and disease.Nature reviews. Genetics · 2015Review
- Diversity of human tRNA genes from the 1000-genomes project.RNA biology · 2013Article
- Coadaptation of isoacceptor tRNA genes and codon usage bias for translation efficiency in Aedes aegypti and Anopheles gambiae.Insect molecular biology · 2011Article
- Different sequence signatures in the upstream regions of plant and animal tRNA genes shape distinct modes of regulation.Nucleic acids research · 2011Article
- Diversity of tRNA genes in eukaryotes.Nucleic acids research · 2006Article
- Modulation of differential transcription of tRNA genes through chromatin organization.The Biochemical journal · 2005Article
- The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment.Nucleic acids research · 2003Article
- A novel TATA-box-binding factor from the silk glands of the mulberry silkworm, Bombyx mori.The Biochemical journal · 2002Article
- Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast.Molecular and cellular biology · 2001Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the contribution of specific TATA-binding protein (TBP)-TATA interactions to the promoter activity of a constitutively expressed silkworm tRNA(C)(Ala) gene and have also asked whether the lack of similar interactions accounts for the low promoter activity of a silk gland-specific tRNA(SG)(Ala) gene. We compared TBP binding, TFIIIB-promoter complex stability (measured by heparin resistance), and in vitro transcriptional activity in a series of mutant tRNA(C)(Ala) promoters and found that specific TBP-TATA contacts are important for TFIIIB-promoter interaction and for transcriptional activity. Although the wild-type tRNA(C)(Ala) promoter contains two functional TBP binding sequences that overlap, the tRNA(SG)(Ala) promoter lacks any TBP binding site in the corresponding region. This feature appears to account for the inefficiency of the tRNA(SG)(Ala) promoter since provision of either of the wild-type TATA sequences derived from the tRNA(C)(Ala) promoter confers robust transcriptional activity. Transcriptional impairment of the wild-type tRNA(SG)(Ala) gene is not due to reduced incorporation of TBP into transcription complexes since both the tRNA(C)(Ala) and tRNA(SG)(Ala) promoters form transcription complexes that contain the same amount of TBP. Thus, the deleterious consequences of the lack of appropriate TBP-TATA contacts in the tRNA(SG)(Ala) promoter must come from failure to incorporate some other essential transcription factor(s) or to stabilize the complete complex in an active conformation.
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