ArticleMolecular and cellular biology2000
Target specificity of the endonuclease from the Xenopus laevis non-long terminal repeat retrotransposon, Tx1L.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Diversity and Evolution of DNA Transposons Targeting Multicopy Small RNA Genes from Actinopterygian Fish.Biology · 2022Article
- A Field Guide to Eukaryotic Transposable Elements.Annual review of genetics · 2020Review
- A New Class of SINEs with snRNA Gene-Derived Heads.Genome biology and evolution · 2015Article
- The diversity of retrotransposons and the properties of their reverse transcriptases.Virus research · 2008Review
- Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies.Nucleic acids research · 2007Article
- Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.Nucleic acids research · 2007Article
- Identification of insertion hot spots for non-LTR retrotransposons: computational and biochemical application to Entamoeba histolytica.Nucleic acids research · 2006Article
- R2 target-primed reverse transcription: ordered cleavage and polymerization steps by protein subunits asymmetrically bound to the target DNA.Molecular and cellular biology · 2005Article
- Telomere-specific non-LTR retrotransposons and telomere maintenance in the silkworm, Bombyx mori.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease.Eukaryotic cell · 2004Article
- Integration of the 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination.Nucleic acids research · 2004Article
- Targeted nuclear import of open reading frame 1 protein is required for in vivo retrotransposition of a telomere-specific non-long terminal repeat retrotransposon, SART1.Molecular and cellular biology · 2004Article
- Dasheng: a recently amplified nonautonomous long terminal repeat element that is a major component of pericentromeric regions in rice.Genetics · 2002Article
- Transplantation of target site specificity by swapping the endonuclease domains of two LINEs.The EMBO journal · 2002Article
- Human L1 retrotransposition: cis preference versus trans complementation.Molecular and cellular biology · 2001Article
- Sequence-specific recognition and cleavage of telomeric repeat (TTAGG)(n) by endonuclease of non-long terminal repeat retrotransposon TRAS1.Molecular and cellular biology · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elements of the Tx1L family are non-long terminal repeat retrotransposons (NLRs) that are dispersed in the genome of Xenopus laevis. Essentially all genomic copies of Tx1L are found inserted at a specific site within another family of transposable elements (Tx1D). This suggests that Tx1L is a site-specific retrotransposon. Like many (but not all) other NLRs, the Xenopus element encodes an apparent endonuclease that is related in sequence to the apurinic-apyrimidinic endonucleases that participate in DNA repair. This enzyme is thought to introduce the single-strand break in target DNA that initiates transposition by the target-primed reverse transcription (TPRT) mechanism. To explore the issue of target specificity more fully, we expressed the polypeptide encoded by the endonuclease domain of open reading frame 2 from Tx1L (Tx1L EN) and characterized its cleavage capabilities. This endonuclease makes a specific nick in the bottom strand precisely at one end of the presumed Tx1L target duplication. Because this activity leaves a 5'-phosphate and 3'-hydroxyl at the nick, it has the location and chemistry required to initiate new insertion events by TPRT. Tx1L EN does not make a specific cut at a preferred target site for Tx1D elements, ruling out the alternative possibility that the composite Tx1L-Tx1D element moves as a unit under the control of functions encoded by Tx1L. Further characterization revealed that the endonuclease remains active for many hours at room temperature and that it is capable of enzymatic turnover. Scanning substitution mutagenesis located the recognition site for Tx1L EN within 10 bp surrounding the primary nick site. Implications of these features for natural transposition events are discussed.
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