ArticleThe Journal of cell biology2000
Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 219 citations in OpenAlex.
- Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.The Journal of cell biology · 2024Article
- Dissection of the role of a Src homology 3 domain in the evolution of binding preference of paralogous proteins.Genetics · 2024Article
- The Arp2/3 complex promotes periodic removal of Pak1-mediated negative feedback to facilitate anticorrelated Cdc42 oscillations.bioRxiv : the preprint server for biology · 2023Article
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- Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS.Nature communications · 2022Article
- Par-3 family proteins in cell polarity & adhesion.The FEBS journal · 2022Review
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- Effects of Mutations in the Phenamacril-Binding Site ofACS omega · 2020Article
- The cytoskeletal motor proteins Dynein and MyoV direct apical transport of Crumbs.Developmental biology · 2020Article
- Human myosin 1e tail but not motor domain replaces fission yeast Myo1 domains to support myosin-I function during endocytosis.Experimental cell research · 2019Article
- Article
- Direct measurement of cortical force generation and polarization in a living parasite.Molecular biology of the cell · 2017Article
- A Computational Model of YAP/TAZ Mechanosensing.Biophysical journal · 2016Article
- An Engineered Minimal WASP-Myosin Fusion Protein Reveals Essential Functions for Endocytosis.Developmental cell · 2015Article
- Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Evolution of the SH3 Domain Specificity Landscape in Yeasts.PloS one · 2015Article
- Arp2/3 complex function in the epidermis.Tissue barriers · 2014Article
- PakD, a putative p21-activated protein kinase in Dictyostelium discoideum, regulates actin.Eukaryotic cell · 2014Article
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
27 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The generation of cortical actin filaments is necessary for processes such as cell motility and cell polarization. Several recent studies have demonstrated that Wiskott-Aldrich syndrome protein (WASP) family proteins and the actin-related protein (Arp) 2/3 complex are key factors in the nucleation of actin filaments in diverse eukaryotic organisms. To identify other factors involved in this process, we have isolated proteins that bind to Bee1p/Las17p, the yeast WASP-like protein, by affinity chromatography and mass spectroscopic analysis. The yeast type I myosins, Myo3p and Myo5p, have both been identified as Bee1p-interacting proteins. Like Bee1p, these myosins are essential for cortical actin assembly as assayed by in vitro reconstitution of actin nucleation sites in permeabilized yeast cells. Analysis using this assay further demonstrated that the motor activity of these myosins is required for the polymerization step, and that actin polymerization depends on phosphorylation of myosin motor domain by p21-activated kinases (PAKs), downstream effectors of the small guanosine triphosphatase, Cdc42p. The type I myosins also interact with the Arp2/3 complex through a sequence at the end of the tail domain homologous to the Arp2/3-activating region of WASP-like proteins. Combined deletions of the Arp2/3-interacting domains of Bee1p and the type I myosins abolish actin nucleation sites at the cortex, suggesting that these proteins function redundantly in the activation of the Arp2/3 complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.