ArticleThe Journal of cell biology2000
A cell-free system for regulated exocytosis in PC12 cells.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 104 citations in OpenAlex.
- A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane.Structure (London, England : 1993) · 2026Article
- Cryo-electron tomography pipeline for plasma membranes.Nature communications · 2025Article
- Identifying Biological and Biophysical Features of Different Maturation States of α-Synuclein Amyloid Fibrils.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.Nature communications · 2021Article
- A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.The Journal of general physiology · 2019Review
- Early Stage Alpha-Synuclein Amyloid Fibrils are Reservoirs of Membrane-Binding Species.Scientific reports · 2019Article
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face.Scientific reports · 2017Article
- AFM/TIRF force clamp measurements of neurosecretory vesicle tethers reveal characteristic unfolding steps.PloS one · 2017Article
- The Nanoscale Observation of the Three-Dimensional Structures of Neurosynapses, Membranous Conjunctions Between Cultured Hippocampal Neurons and Their Significance in the Development of Epilepsy.Molecular neurobiology · 2016Article
- The tetraspanin web revisited by super-resolution microscopy.Scientific reports · 2015Article
- Cholesterol modulates CFTR confinement in the plasma membrane of primary epithelial cells.Biophysical journal · 2015Article
- The extracellular δ-domain is essential for the formation of CD81 tetraspanin webs.Biophysical journal · 2014Article
- Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.Journal of chemical biology · 2014Article
- Article
- Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy.Journal of biomaterials and nanobiotechnology · 2012Article
- Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding.Methods in cell biology · 2012Article
- Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function.Molecular biology of the cell · 2011Article
- The t-SNARE complex: a close up.Cellular and molecular neurobiology · 2010Review
- The platelet actin cytoskeleton associates with SNAREs and participates in alpha-granule secretion.Biochemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a cell-free system for regulated exocytosis in the PC12 neuroendocrine cell line. Secretory vesicles were preloaded with acridine orange in intact cells, and the cells were sonicated to produce flat, carrier-supported plasma membrane patches with attached vesicles. Exocytosis resulted in the release of acridine orange which was visible as a disappearance of labeled vesicles and, under optimal conditions, produced light flashes by fluorescence dequenching. Exocytosis in vitro requires cytosol and Ca(2+) at concentrations in the micromolar range, and is sensitive to Tetanus toxin. Imaging of membrane patches at diffraction- limited resolution revealed that 42% of docked granules were released in a Ca(2+)-dependent manner during 1 min of stimulation. Electron microscopy of membrane patches confirmed the presence of dense-core vesicles. Imaging of membrane patches by atomic force microscopy revealed the presence of numerous particles attached to the membrane patches which decreased in number upon stimulation. Thus, exocytotic membrane fusion of single vesicles can be monitored with high temporal and spatial resolution, while providing access to the site of exocytosis for biochemical and molecular tools.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.