ArticleThe Journal of cell biology2000
An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B" spliceosome proteins.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Biallelic variants in SNUPN cause a limb girdle muscular dystrophy with myofibrillar-like features.Brain : a journal of neurology · 2024Article
- Comparative Proteomic Analysis of Drug Trichosanthin Addition to BeWo Cell Line.Molecules (Basel, Switzerland) · 2022Article
- An in vitro reconstituted U1 snRNP allows the study of the disordered regions of the particle and the interactions with proteins and ligands.Nucleic acids research · 2021Article
- Domain Requirements and Genetic Interactions of the Mud1 Subunit of theG3 (Bethesda, Md.) · 2019Article
- Avian reovirus sigmaA localizes to the nucleolus and enters the nucleus by a nonclassical energy- and carrier-independent pathway.Journal of virology · 2009Article
- ATP modulates PTEN subcellular localization in multiple cancer cell lines.Human molecular genetics · 2008Article
- Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors.Retrovirology · 2008Article
- Article
- prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast.Nature structural & molecular biology · 2007Article
- Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies.Molecular biology of the cell · 2006Article
- ALADINI482S causes selective failure of nuclear protein import and hypersensitivity to oxidative stress in triple A syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Importin alpha can migrate into the nucleus in an importin beta- and Ran-independent manner.The EMBO journal · 2002Article
- Energetic communication between mitochondria and nucleus directed by catalyzed phosphotransfer.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Mechanisms controlling subcellular localization of the G(1) cyclins Cln2p and Cln3p in budding yeast.Molecular and cellular biology · 2001Article
- Disruption of the FG nucleoporin NUP98 causes selective changes in nuclear pore complex stoichiometry and function.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear import of the two uracil-rich small nuclear ribonucleoprotein (U snRNP) components U1A and U2B" is mediated by unusually long and complex nuclear localization signals (NLSs). Here we investigate nuclear import of U1A and U2B" in vitro and demonstrate that it occurs by an active, saturable process. Several lines of evidence suggest that import of the two proteins occurs by an import mechanism different to those characterized previously. No cross competition is seen with a variety of previously studied NLSs. In contrast to import mediated by members of the importin-beta family of nucleocytoplasmic transport receptors, U1A/U2B" import is not inhibited by either nonhydrolyzable guanosine triphosphate (GTP) analogues or by a mutant of the GTPase Ran that is incapable of GTP hydrolysis. Adenosine triphosphate is capable of supporting U1A and U2B" import, whereas neither nonhydrolyzable adenosine triphosphate analogues nor GTP can do so. U1A and U2B" import in vitro does not require the addition of soluble cytosolic proteins, but a factor or factors required for U1A and U2B" import remains tightly associated with the nuclear fraction of conventionally permeabilized cells. This activity can be solubilized in the presence of elevated MgCl(2). These data suggest that U1A and U2B" import into the nucleus occurs by a hitherto uncharacterized mechanism.
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