ArticleThe Journal of cell biology2000
Electron tomography reveals posttranscriptional binding of pre-mRNPs to specific fibers in the nucleoplasm.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- The Chironomus tentans genome sequence and the organization of the Balbiani ring genes.BMC genomics · 2014Article
- Review
- Balbiani ring mRNPs diffuse through and bind to clusters of large intranuclear molecular structures.Biophysical journal · 2010Article
- Single-molecule dynamics of nuclear mRNA.F1000 biology reports · 2009Article
- Discontinuous movement of mRNP particles in nucleoplasmic regions devoid of chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The antibody zalutumumab inhibits epidermal growth factor receptor signaling by limiting intra- and intermolecular flexibility.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- In-silico prediction and observations of nuclear matrix attachment.Cellular & molecular biology letters · 2006Article
- P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner.Molecular biology of the cell · 2005Article
- The growing pre-mRNA recruits actin and chromatin-modifying factors to transcriptionally active genes.Genes & development · 2005Article
- Hrp59, an hnRNP M protein in Chironomus and Drosophila, binds to exonic splicing enhancers and is required for expression of a subset of mRNAs.The Journal of cell biology · 2005Article
- Nephrin strands contribute to a porous slit diaphragm scaffold as revealed by electron tomography.The Journal of clinical investigation · 2004Article
- Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells.The Journal of cell biology · 2004Article
- Nephrin promotes cell-cell adhesion through homophilic interactions.The American journal of pathology · 2003Article
- PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression.Molecular and cellular biology · 2003Article
- An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- PSF and p54nrb bind a conserved stem in U5 snRNA.RNA (New York, N.Y.) · 2002Article
- Evidence for a posttranscriptional role of a TFIIICalpha-like protein in Chironomus tentans.Molecular biology of the cell · 2002Article
- Amino acid substitutions of coiled-coil protein Tpr abrogate anchorage to the nuclear pore complex but not parallel, in-register homodimerization.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using electron tomography, we have analyzed whether the Balbiani ring (BR) pre-mRNP particles in transit from the gene to the nuclear pore complex (NPC) are bound to any structure that could impair free diffusion through the nucleoplasm. We show that one-third of the BR particles are in contact with thin connecting fibers (CFs), which in some cases merge into large fibrogranular clusters. The CFs have a specific protein composition different from that of BR particles, as shown by immuno-EM. Moreover, we have identified hrp65 as one of the protein components of the CFs. The sequencing of hrp65 cDNA reveals similarities with hnRNP proteins and splicing factors. However, hrp65 is likely to have a different function because it does not bind to nascent pre-mRNA and is not part of the pre-mRNP itself. Taken together, our observations indicate that pre-mRNPs are not always freely diffusible in the nucleoplasm but interact with fibers of specific structure and composition, which implies that some of the posttranscriptional events that the pre-mRNPs undergo before reaching the NPC occur in a bound state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.