ArticleCell proliferation1999
Spontaneous apoptosis and expression of cell surface heat-shock proteins in cultured EL-4 lymphoma cells.
Article in Cell proliferation, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- Review
- Review
- Heat Shock Protein 60 in Hepatocellular Carcinoma: Insights and Perspectives.Frontiers in molecular biosciences · 2020Review
- Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease.Journal of neuroinflammation · 2014Article
- Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Screening of multiple myeloma by polyclonal rabbit anti-human plasmacytoma cell immunoglobulin.PloS one · 2013Article
- Heat-shock-induced cellular responses to temperature elevations occurring during orthopaedic cutting.Journal of the Royal Society, Interface · 2012Article
- The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.Journal of veterinary science · 2011Article
- The role of damage associated molecular pattern molecules in acetaminophen-induced liver injury in mice.Toxicology letters · 2010Article
- Chlamydia trachomatis infection and anti-Hsp60 immunity: the two sides of the coin.PLoS pathogens · 2009Review
- S-layer, surface-accessible, and concanavalin A binding proteins of Methanosarcina acetivorans and Methanosarcina mazei.Journal of proteome research · 2009Article
- Heat shock proteins: linking danger and pathogen recognition.Medical microbiology and immunology · 2008Review
- Heat shock protein-90-alpha, a prolactin-STAT5 target gene identified in breast cancer cells, is involved in apoptosis regulation.Breast cancer research : BCR · 2008Article
- Extracellular heat shock protein 90: a role for a molecular chaperone in cell motility and cancer metastasis.Cancer science · 2007Review
- Expression of 60 kDa heat shock protein (Hsp60) on plasma membrane of Daudi cells.Molecular and cellular biochemistry · 2004Article
- Lipid interaction differentiates the constitutive and stress-induced heat shock proteins Hsc70 and Hsp70.Cell stress & chaperones · 2002Article
- Chaperonin 60 unfolds its secrets of cellular communication.Cell stress & chaperones · 2002Review
- Translocation of cytoplasmic HSP70 onto the surface of EL-4 cells during apoptosis.Cell proliferation · 2002Article
- Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity.Cell stress & chaperones · 2002Article
- Involvement of heat shock proteins in the phenomenon of cell protection against apoptosis mediated by inhibitors of plasma membrane chlorine channels.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of heat-shock proteins (HSPs) is enhanced in stressed cells and can protect cells from stress-induced injury. However, existing data about the relationship between apoptosis and HSP expression is contradictory. In this paper, a mouse lymphoma cell death model system is used to detect simultaneously both the process of apoptosis and the level of HSP expression. The model was established after discovering that spontaneous apoptosis and spontaneous cell surface HSP expression occurs in EL-4 mouse lymphoma cells during normal optimal culture conditions. The data show that apoptotic EL-4 cells had higher levels of hsp25, hsp60, hsp70 and hsp90 exposed on the plasma membrane surface than viable cells. The level of surface HSPs was found to increase through several stages of early and late apoptotic death as measured by flow cytometry, with the highest levels observed during the loss of cell membrane phospholipid asymmetry. Heat shock and actinomycin D significantly increased the proportion of apoptotic cells in culture. However, hyperthermia only stimulated a weak and temporary increase in surface HSP expression, whereas actinomycin D strongly elevated the level of surface and intracellular HSPs, particularly in live cells. These results show an associative relationship between apoptosis and HSP expression. The relationship between the progression of cell death and HSP expression suggests a role for membrane HSP expression in programmed cell death.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.