ArticlePlant molecular biology1999
Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nia1 promoter.
Article in Plant molecular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Ammonium-nitrate mixtures dominated by NHFrontiers in plant science · 2023Article
- Orphan Genes in Crop Improvement: Enhancing Potato Tuber Protein without Impacting Yield.Plants (Basel, Switzerland) · 2022Article
- Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica.Applied microbiology and biotechnology · 2020Article
- Origin Recognition Complex (ORC) Evolution Is Influenced by Global Gene Duplication/Loss Patterns in Eukaryotic Genomes.Genome biology and evolution · 2020Article
- Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae.International journal of molecular sciences · 2020Article
- The Bacterial Phytoene Desaturase-Encoding Gene (Metabolites · 2019Article
- Dual positive and negative control of Chlamydomonas PII signal transduction protein expression by nitrate/nitrite and NO via the components of nitric oxide cycle.BMC plant biology · 2018Article
- Article
- Characterization of a Mutant Deficient for Ammonium and Nitric Oxide Signalling in the Model System Chlamydomonas reinhardtii.PloS one · 2016Article
- Characterization of unusual truncated hemoglobins of Chlamydomonas reinhardtii suggests specialized functions.Planta · 2015Article
- The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.BMC biotechnology · 2015Article
- Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures.Biotechnology for biofuels · 2015Article
- Understanding nitrate assimilation and its regulation in microalgae.Frontiers in plant science · 2015Review
- Promoter trapping in microalgae using the antibiotic paromomycin as selective agent.Marine drugs · 2012Article
- Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis.Applied microbiology and biotechnology · 2011Article
- A soluble guanylate cyclase mediates negative signaling by ammonium on expression of nitrate reductase in Chlamydomonas.The Plant cell · 2010Article
- Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii.The Journal of biological chemistry · 2009Article
- Nitrate assimilation in Chlamydomonas.Eukaryotic cell · 2008Review
- Nitrite acts as a transcriptome signal at micromolar concentrations in Arabidopsis roots.Plant physiology · 2007Article
- Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas.The Plant cell · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NAD(P)H nitrate reductase (NR) from Chlamydomonas reinhardtii is encoded by the structural gene Nia1. Numerous data from the literature indicate that this enzyme is submitted to complex regulation mechanisms involving multiple controls at transcriptional and post-transcriptional levels. To specifically investigate the regulation of the Nia1 gene at the transcriptional level, NR+ and NR- transformed cells harbouring the Nia1:Ars construct (Nia1 promoter fused to the arylsulfatase (ARS)-encoding Ars reporter gene) were cultivated under various experimental conditions and the ARS activities were recorded. ARS levels were very low in cells grown in the presence of NH4Cl and dramatically increased on agar medium deprived of any nitrogen source or containing nitrate, nitrite, urea, arginine or glutamine. Compared to nitrogen-free medium, a slight positive effect of nitrate in the NR+ strain and a significant negative effect of nitrite in both NR+ and NR- strains were observed. The ARS activities were high in the light and very low in the dark or in the light in the presence of DCMU, indicating that Nia1 transcription is strikingly dependent on photosynthetic activity. Acetate used as a carbon source in the dark did not substitute for light in stimulating Nia1:Ars expression. Inactivation of NR by tungstate treatment of the NR+ strain resulted in a dramatic increase of ARS level suggesting that in Chlamydomonas, like in higher plants, active NR negatively regulates the transcription of the NR structural gene. Deleting the major part of the Nia1 leader sequence still present in the chimeric gene resulted in a decrease of ARS level but did not modify the regulation pattern.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.