ArticleOncogene2000
Replacing two conserved tyrosines of the EphB2 receptor with glutamic acid prevents binding of SH2 domains without abrogating kinase activity and biological responses.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 123 citations in OpenAlex.
- Large-scale analysis of the N-terminal regulatory elements of the kinase domain in plant Receptor-like kinase family.BMC plant biology · 2024Article
- Article
- The Roles of EphB2 in Cancer.Frontiers in cell and developmental biology · 2022Review
- Regulation of the EphA2 receptor intracellular region by phosphomimetic negative charges in the kinase-SAM linker.Nature communications · 2021Article
- Review
- The EphA4 Signaling is Anti-catabolic in Synoviocytes but Pro-anabolic in Articular Chondrocytes.Calcified tissue international · 2020Article
- Exploring protein phosphorylation by combining computational approaches and biochemical methods.Computational and structural biotechnology journal · 2020Article
- A single phosphoacceptor residue in BGLF3 is essential for transcription of Epstein-Barr virus late genes.PLoS pathogens · 2019Article
- A novel miR17/protein tyrosine phosphatase-oc/EphA4 regulatory axis of osteoclast activity.Archives of biochemistry and biophysics · 2018Review
- Activation of EphB2 Forward Signaling Enhances Memory Consolidation.Cell reports · 2018Article
- Coupled regulation by the juxtamembrane and sterile α motif (SAM) linker is a hallmark of ephrin tyrosine kinase evolution.The Journal of biological chemistry · 2018Article
- EphB4: A promising target for upper aerodigestive malignancies.Biochimica et biophysica acta. Reviews on cancer · 2018Review
- Phosphorylation of the Bovine Papillomavirus E2 Protein on Tyrosine Regulates Its Transcription and Replication Functions.Journal of virology · 2017Article
- Phosphorylation Alters Oestrogen Receptor β-Mediated Transcription in Neurones.Journal of neuroendocrinology · 2015Article
- EphB/ephrinB signaling in cell adhesion and migration.Molecules and cells · 2015Review
- EphB2 regulates contact-dependent and contact-independent signaling to control platelet function.Blood · 2015Article
- A phosphotyrosine switch for cargo sequestration at clathrin-coated buds.The Journal of biological chemistry · 2014Article
- Regulation of cell differentiation by Eph receptor and ephrin signaling.Cell adhesion & migration · 2014Review
- Eph receptor signaling and ephrins.Cold Spring Harbor perspectives in biology · 2013Review
- Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.Molecular biology of the cell · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Eph receptor tyrosine kinases play key roles in pattern formation during embryonic development, but little is known about the mechanisms by which they elicit specific biological responses in cells. Here, we investigate the role of tyrosines 605 and 611 in the juxtamembrane region of EphB2, because they are conserved Eph receptor autophosphorylation sites and demonstrated binding sites for the SH2 domains of multiple signaling proteins. Mutation of tyrosines 605 and 611 to phenylalanine impaired EphB2 kinase activity, complicating analysis of their function as SH2 domain binding sites and their contribution to EphB2-mediated signaling. In contrast, mutation to the negatively charged glutamic acid disrupted SH2 domain binding without reducing EphB2 kinase activity. By using a panel of EphB2 mutants, we found that kinase activity is required for the changes in cell-matrix and cell - cell adhesion, cytoskeletal organization, and activation of mitogen-activated protein (MAP) kinases elicited by EphB2 in transiently transfected cells. Instead, the two juxtamembrane SH2 domain binding sites were dispensable for these effects. These results suggest that phosphorylation of tyrosines 605 and 611 is critical for EphB2-mediated cellular responses because it regulates EphB2 kinase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.