ArticleOncogene2000
Non-activated p53 co-localizes with sites of transcription within both the nucleoplasm and the nucleolus.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy.BMC bioinformatics · 2024Article
- Review
- Aspirin Prevention of Colorectal Cancer: Focus on NF-κB Signalling and the Nucleolus.Biomedicines · 2017Review
- High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation.Nucleic acids research · 2015Article
- Effects of lead on the morphology and structure of the nucleolus in the root tip meristematic cells of Allium cepa L.International journal of molecular sciences · 2014Article
- Nucleolar control of p53: a cellular Achilles' heel and a target for cancer therapy.Cellular and molecular life sciences : CMLS · 2014Review
- Emerging roles of the nucleolus in regulating the DNA damage response: the noncanonical DNA repair enzyme APE1/Ref-1 as a paradigmatical example.Antioxidants & redox signaling · 2014Review
- Nucleolus as an oxidative stress sensor in the yeast Saccharomyces cerevisiae.Redox report : communications in free radical research · 2010Article
- Nucleoplasmic mobilization of nucleostemin stabilizes MDM2 and promotes G2-M progression and cell survival.Journal of cell science · 2008Article
- Nucleolar trafficking of nucleostemin family proteins: common versus protein-specific mechanisms.Molecular and cellular biology · 2007Article
- Energy-dependent nucleolar localization of p53 in vitro requires two discrete regions within the p53 carboxyl terminus.Oncogene · 2007Article
- Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm.Journal of cell science · 2006Article
- Differential intranuclear organization of transcription factors Sp1 and Sp3.Molecular biology of the cell · 2005Article
- Nucleolar sequestration of RelA (p65) regulates NF-kappaB-driven transcription and apoptosis.Molecular and cellular biology · 2005Article
- A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin.The Journal of cell biology · 2005Article
- p14ARF is a component of the p53 response following ionizing irradiation of normal human fibroblasts.Oncogene · 2004Article
- Automatic and quantitative measurement of protein-protein colocalization in live cells.Biophysical journal · 2004Article
- Impaired p53 expression, function, and nuclear localization in calreticulin-deficient cells.Molecular biology of the cell · 2004Article
- Article
- p53 is a chromatin accessibility factor for nucleotide excision repair of DNA damage.The EMBO journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The p53 tumour suppressor functions as a sensor of genotoxic stress and, once activated, induces cell growth arrest or apoptosis. The precise intranuclear localization of latent p53 protein in non-stressed cells is unknown. Such information is essential in order to understand how relatively few molecules of p53 can detect and respond to DNA damage. Here we present the first detailed supramolecular localization of p53 in the nuclei of cells under normal conditions of growth. We show that soluble, non-bound p53 is released by permeabilization, leaving structurally bound p53 in both the nucleus and nucleolus. In situ biochemical studies reveal (i) that nuclear-bound p53 is tethered by RNA (directly or indirectly) and (ii) that a sub-population of nuclear-bound p53 co-localizes with sites of RNA synthesis. Transcriptional co-localization appeared to be independent of p53 conformation but dependent upon its quaternary structure. In the nucleolus p53 was observed at sites of rRNA synthesis and also adjacent to such sites. In contrast, nucleolar hdm-2 (shown by others to complex p53 and 5S RNA) was excluded from sites of rRNA synthesis. Our discovery that p53 is physically linked with sites of transcription may explain how relatively few p53 protein molecules can monitor genetic stress and respond preferentially to damage of actively transcribed genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.