ArticleOncogene2000
Roles for the protein tyrosine phosphatase SHP-2 in cytoskeletal organization, cell adhesion and cell migration revealed by overexpression of a dominant negative mutant.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 92 citations in OpenAlex.
- Mechanosensor Piezo1 mediates bimodal patterns of intracellular calcium and FAK signaling.The EMBO journal · 2022Article
- Inner retinal injury in experimental glaucoma is prevented upon AAV mediated Shp2 silencing in a caveolin dependent manner.Theranostics · 2021Article
- Noonan Syndrome-Associated SHP2 Dephosphorylates GluN2B to Regulate NMDA Receptor Function.Cell reports · 2018Article
- Cytotoxic activity of Shp2 inhibitor fumosorinone in human cancer cells.Oncology letters · 2018Article
- Shp2-Mitogen-Activated Protein Kinase Signaling Drives Proliferation during Zebrafish Embryo Caudal Fin Fold Regeneration.Molecular and cellular biology · 2018Article
- Protein tyrosine phosphatase Shp2 deficiency in podocytes attenuates lipopolysaccharide-induced proteinuria.Scientific reports · 2017Article
- New and Unexpected Biological Functions for the Src-Homology 2 Domain-Containing Phosphatase SHP-2 in the Gastrointestinal Tract.Cellular and molecular gastroenterology and hepatology · 2016Review
- SHP-2 Mediates Cryptosporidium parvum Infectivity in Human Intestinal Epithelial Cells.PloS one · 2015Article
- SHP2 positively regulates TGFβ1-induced epithelial-mesenchymal transition modulated by its novel interacting protein Hook1.The Journal of biological chemistry · 2014Article
- The tyrosine phosphatase SHP2 regulates focal adhesion kinase to promote EGF-induced lamellipodia persistence and cell migration.Molecular cancer research : MCR · 2013Article
- Gab2 regulates cytoskeletal organization and migration of mammary epithelial cells by modulating RhoA activation.Molecular biology of the cell · 2011Article
- [Expression and its clinical significance of SHP2 in non-small cell lung cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2010Article
- SHP-2 expression negatively regulates NK cell function.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Protein tyrosine phosphatase SHP-2: a proto-oncogene product that promotes Ras activation.Cancer science · 2009Review
- Molecular mechanism for SHP2 in promoting HER2-induced signaling and transformation.The Journal of biological chemistry · 2009Article
- Noonan syndrome/leukemia-associated gain-of-function mutations in SHP-2 phosphatase (PTPN11) enhance cell migration and angiogenesis.The Journal of biological chemistry · 2009Article
- Potential molecular mechanism for c-Src kinase-mediated regulation of intestinal cell migration.The Journal of biological chemistry · 2008Article
- CaMK-II promotes focal adhesion turnover and cell motility by inducing tyrosine dephosphorylation of FAK and paxillin.Cell motility and the cytoskeleton · 2008Article
- Regulation of RhoA-dependent ROCKII activation by Shp2.The Journal of cell biology · 2008Article
- Nuclear localization of the ERK MAP kinase mediated by Drosophila alphaPS2betaPS integrin and importin-7.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SHP-2, a SRC homology 2 domain-containing protein tyrosine phosphatase, mediates activation of Ras and mitogen-activated protein kinase by various mitogens and cell adhesion. Inhibition of endogenous SHP-2 by overexpression of a catalytically inactive (dominant negative) mutant in Chinese hamster ovary cells or Rat-1 fibroblasts has now been shown to induce a marked change in cell morphology (from elongated to less polarized) that is accompanied by substantial increases in the numbers of actin stress fibers and focal adhesion contacts. Overexpression of the SHP-2 mutant also increased the strength of cell-substratum adhesion and resulted in hyperphosphorylation of SHPS-1, a substrate of SHP-2 that contributes to cell adhesion-induced signaling. Inhibition of SHP-2 also markedly increased the rate of cell attachment to and cell spreading on extracellular matrix proteins such as fibronectin and vitronectin, effects that were accompanied by enhancement of adhesion-induced tyrosine phosphorylation of paxillin and p130Cas. In addition, cell migration mediated by fibronectin or vitronectin, but not that induced by insulin, was impaired by overexpression of the SHP-2 mutant. These results suggest that SHP-2 plays an important role in the control of cell shape by contributing to cytoskeletal organization, and that it is an important regulator of integrin-mediated cell adhesion, spreading, and migration as well as of tyrosine phosphorylation of focal adhesion contact-associated proteins.
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