ArticleThe Journal of clinical investigation2000
Requirement of aquaporin-1 for NaCl-driven water transport across descending vasa recta.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 122 citations in OpenAlex.
- Aquaporin-1 and Osmosis: From Physiology to Precision in Peritoneal Dialysis.Journal of the American Society of Nephrology : JASN · 2024Review
- Renal water transport in health and disease.Pflugers Archiv : European journal of physiology · 2022Review
- Aquaporins in Cancer Biology.Frontiers in oncology · 2022Review
- Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway.FEBS open bio · 2021Article
- The Importance of Aquaporin 1 in Pancreatitis and Its Relation to the CFTR ClFrontiers in physiology · 2018Article
- Physiological role of aquaporin 5 in salivary glands.Pflugers Archiv : European journal of physiology · 2016Review
- Expression of aquaporin1, a water channel protein, in cytoplasm is negatively correlated with prognosis of breast cancer patients.Oncotarget · 2016Article
- The Trafficking of the Water Channel Aquaporin-2 in Renal Principal Cells-a Potential Target for Pharmacological Intervention in Cardiovascular Diseases.Frontiers in pharmacology · 2016Review
- Effect of intense pulsed light on the expression of aquaporin 3 in rat skin.Lasers in medical science · 2015Article
- Isolation and perfusion of rat inner medullary vasa recta.American journal of physiology. Renal physiology · 2015Article
- Transplantation of cultured rhesus monkey vascular endothelial cells to allogeneic cornea concomitant with stripping of Descemet's membrane.Indian journal of ophthalmology · 2015Article
- Targeted delivery of solutes and oxygen in the renal medulla: role of microvessel architecture.American journal of physiology. Renal physiology · 2014Review
- Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats.Journal of physiology and biochemistry · 2014Article
- Aquaporins: important but elusive drug targets.Nature reviews. Drug discovery · 2014Review
- Comparative physiology and architecture associated with the mammalian urine concentrating mechanism: role of inner medullary water and urea transport pathways in the rodent medulla.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Review
- Molecular biology of water and salt regulation in the kidney.Cellular and molecular life sciences : CMLS · 2012Review
- Aquaporins in clinical medicine.Annual review of medicine · 2012Review
- Effect of Atractylodes macrocephala on Hypertonic Stress-Induced Water Channel Protein Expression in Renal Collecting Duct Cells.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Aquaporins as gas channels.Pflugers Archiv : European journal of physiology · 2011Review
- Modeling transport in the kidney: investigating function and dysfunction.American journal of physiology. Renal physiology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Deletion of AQP1 in mice results in diminished urinary concentrating ability, possibly related to reduced NaCl- and urea gradient-driven water transport across the outer medullary descending vasa recta (OMDVR). To quantify the role of AQP1 in OMDVR water transport, we measured osmotically driven water permeability in vitro in microperfused OMDVR from wild-type, AQP1 heterozygous, and AQP1 knockout mice. OMDVR diameters in AQP1(-/-) mice were 1.9-fold greater than in AQP1(+/+) mice. Osmotic water permeability (P(f)) in response to a 200 mM NaCl gradient (bath > lumen) was reduced about 2-fold in AQP1(+/-) mice and by more than 50-fold in AQP1(-/-) mice. P(f) increased from 1015 to 2527 microm/s in AQP1(+/+) mice and from 22 to 1104 microm/s in AQP1(-/-) mice when a raffinose rather than an NaCl gradient was used. This information, together with p-chloromercuribenzenesulfonate inhibition measurements, suggests that nearly all NaCl-driven water transport occurs by a transcellular route through AQP1, whereas raffinose-driven water transport also involves a parallel, AQP1-independent, mercurial-insensitive pathway. Interestingly, urea was also able to drive water movement across the AQP1-independent pathway. Diffusional permeabilities to small hydrophilic solutes were comparable in AQP1(+/+) and AQP1(-/-) mice but higher than those previously measured in rats. In a mathematical model of the medullary microcirculation, deletion of AQP1 resulted in diminished concentrating ability due to enhancement of medullary blood flow, partially accounting for the observed urine-concentrating defect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.