ArticleThe Biochemical journal2000
Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 56 citations in OpenAlex.
- Differentiation of Promonocytic U937 Cells to Monocytes Is Associated with Reduced Mitochondrial Transport of Ascorbic Acid.Oxidative medicine and cellular longevity · 2018Article
- Intramitochondrial Ascorbic Acid Enhances the Formation of Mitochondrial Superoxide Induced by Peroxynitrite via a CaInternational journal of molecular sciences · 2017Article
- Assessing Oxidative Stress in Tumors by Measuring the Rate of Hyperpolarized [1-13C]Dehydroascorbic Acid Reduction Using 13C Magnetic Resonance Spectroscopy.The Journal of biological chemistry · 2017Article
- Old Things New View: Ascorbic Acid Protects the Brain in Neurodegenerative Disorders.International journal of molecular sciences · 2015Review
- Assessing the reductive capacity of cells by measuring the recycling of ascorbic and lipoic acids.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Inflammation in the vascular bed: importance of vitamin C.Pharmacology & therapeutics · 2008Review
- Constraint-based modeling and kinetic analysis of the Smad dependent TGF-beta signaling pathway.PloS one · 2007Article
- Ascorbate transport and recycling by SH-SY5Y neuroblastoma cells: response to glutamate toxicity.Neurochemical research · 2006Article
- Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.Plant physiology · 2004Article
- Enhancing effects of intracellular ascorbic acid on peroxynitrite-induced U937 cell death are mediated by mitochondrial events resulting in enhanced sensitivity to peroxynitrite-dependent inhibition of complex III and formation of hydrogen peroxide.The Biochemical journal · 2004Article
- Vitamin C and oxidative stress on cultured human keratinocytes.The Canadian journal of plastic surgery = Journal canadien de chirurgie plastique · 2004Article
- Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells.The Biochemical journal · 2001Article
- Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage.The Biochemical journal · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin C plays an important role in neutralizing toxic free radicals formed during oxidative metabolism or UV exposure of human skin. This study was performed to investigate the mechanisms that regulate the homoeostasis of vitamin C in HaCaT cells by identifying the events involved in the transport and in the reduction of dehydroascorbic acid. Dehydroascorbic acid accumulated to a greater extent and faster compared with ascorbic acid; its transport appeared to be mediated by hexose transporters and was entirely distinct from ascorbic acid transport. Dehydroascorbate reductase activity was unaffected by glutathione depletion, although it was sensitive to thiol protein reagents. These observations, as well as the subcellular distribution of this enzymic activity and the cofactor specificity, indicate that thioredoxin reductase and lipoamide dehydrogenase play an important role in this reduction process. HaCaT cells were able to enhance their dehydroascorbic acid reductase activity in response to oxidative stress.
Indexed as
Identifiers
10642526PMC1220802W2071262907What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.