ArticleThe Biochemical journal2000
The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral Streptococci and exhibits candidacidal activity.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- Evaluation of salivary mucins in children with deciduous and mixed dentition: comparative analysis between high and low caries-risk groups.Clinical oral investigations · 2015Trial
- Beyond proteolysis: rational modification of mucin-derived peptidomimetics with enhanced metal-mediated antimicrobial activity.RSC advances · 2026Article
- Review
- Insights into the Chemistry, Structure, and Biological Activity of Human Salivary MUC7 Fragments and Their Cu(II) and Zn(II) Complexes.Inorganic chemistry · 2024Article
- Short chain fatty acids induced the type 1 and type 2 fimbrillin-dependent and fimbrillin-independent initial attachment and colonization of Actinomyces oris monoculture but not coculture with streptococci.BMC microbiology · 2020Article
- Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV).Frontiers in cellular and infection microbiology · 2020Article
- Exploring the role and diversity of mucins in health and disease with special insight into non-communicable diseases.Glycoconjugate journal · 2015Review
- Salivary mucin 19 glycoproteins: innate immune functions in Streptococcus mutans-induced caries in mice and evidence for expression in human saliva.The Journal of biological chemistry · 2015Article
- Salivary mucins in host defense and disease prevention.Journal of oral microbiology · 2015Article
- Group A streptococcus adheres to pharyngeal epithelial cells with salivary proline-rich proteins via GrpE chaperone protein.The Journal of biological chemistry · 2012Article
- An unusual glycoform of human salivary mucin MG2.Clinical oral investigations · 2012Article
- Mucin-bacterial interactions in the human oral cavity and digestive tract.Gut microbes · 2010Article
- Identification of salivary mucin MUC7 binding proteins from Streptococcus gordonii.BMC microbiology · 2009Article
- Mucins in the mucosal barrier to infection.Mucosal immunology · 2008Review
- Host-derived pentapeptide affecting adhesion, proliferation, and local pH in biofilm communities composed of Streptococcus and Actinomyces species.Infection and immunity · 2006Article
- [Expression of antimicrobial peptide MUC7 in kidneys with pyelonephritis].Der Urologe. Ausg. A · 2006Article
- The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to host cells.Infection and immunity · 2005Article
- [Lacrimal gland-associated mucins. Age related production and their role in the pathophysiology of dry eye].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2005Review
- [Tear outflow. Impact of mucins and TFF-peptides].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2004Review
- Human salivary MUC7 mucin peptides: effect of size, charge and cysteine residues on antifungal activity.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
MG2 (the MUC7 gene product) is a low-molecular-mass mucin found in human submandibular/sublingual secretions. This mucin is believed to agglutinate a variety of microbes and thus is considered an important component of the non-immune host defence system in the oral cavity. We have shown that MUC7 can bind to cariogenic strains of Streptococcus mutans and that this binding requires a structural determinant in the N-terminal region. In the present study an expression construct, pNMuc7, encoding the N-terminal 144 amino acids of MUC7 was generated, and the recombinant protein rNMUC7 was expressed in Escherichia coli. Purified rNMUC7 was characterized and the binding of this protein to oral bacteria was investigated in an established assay. The results showed that the recombinant protein bound to S. mutans ATCC 25175 and ATCC 33402, and that alkylation of the two cysteine residues (Cys(45) and Cys(50)) resulted in the complete loss of bacterial binding. This suggests that binding of MUC7 to S. mutans occurs between the N-terminal region of the mucin molecule and the bacterial surface, and that this interaction is dependent on a cysteine-containing domain within this region of MUC7. In addition, the killing activity of rNMUC7 was compared with that of the candidacidal salivary protein histatin 5 in an established Candida albicans (ATCC 44505) blastoconidia killing assay. It was found that the LD(50) values of rNMUC7 and histatin 5 were comparable, and that the recombinant protein displayed significant killing activity at the physiological concentration range of MUC7 in whole saliva. This study is the first to show that the N-terminal region of MUC7 contains a structural determinant for bacterial binding and that this region exhibits candidacidal activity.
Indexed as
Identifiers
10642514PMC1220790W2076283114What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.