ArticleInfection and immunity2000
Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Vaccine approaches for the 'therapeutic management' ofThe veterinary quarterly · 2019Review
- An update on Mycobacterium avium subspecies paratuberculosis antigens and their role in the diagnosis of Johne's disease.World journal of microbiology & biotechnology · 2019Review
- Recombinant fusion protein of Heparin-Binding Hemagglutinin Adhesin and Fibronectin Attachment Protein (rHBHA-FAP) ofGut pathogens · 2019Article
- Article
- Development of vaccines to Mycobacterium avium subsp. paratuberculosis infection.Clinical and experimental vaccine research · 2016Review
- Surface proteome of "Mycobacterium avium subsp. hominissuis" during the early stages of macrophage infection.Infection and immunity · 2012Article
- Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis.Proteome science · 2012Article
- Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis.The Journal of infectious diseases · 2011Article
- Mycobacterium avium subsp. Paratuberculosis and the expression of selected virulence and pathogenesis genes in response to 6°C, 65°c and ph 2.0.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli.Molecular biology international · 2010Article
- A rhodanine agent active against non-replicating intracellular Mycobacterium avium subspecies paratuberculosis.Gut pathogens · 2009Article
- Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients.PloS one · 2009Article
- An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
- A novel multi-antigen virally vectored vaccine against Mycobacterium avium subspecies paratuberculosis.PloS one · 2007Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Proteome and differential expression analysis of membrane and cytosolic proteins from Mycobacterium avium subsp. paratuberculosis strains K-10 and 187.Journal of bacteriology · 2007Article
- Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.Infection and immunity · 2005Article
- Characterization of novel coding sequences specific to Mycobacterium avium subsp. paratuberculosis: implications for diagnosis of Johne's Disease.Journal of clinical microbiology · 2004Article
- Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp. paratuberculosis.Journal of clinical microbiology · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antigens characteristic for Mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (CIE) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent Mycobacterium avium subspecies avium antiserum. Two antigens were present in M. avium subsp. paratuberculosis and not detected in Mycobacterium avium subsp. avium. They were identified as antigens 17 and 20 in a CIE reference system for M. avium subsp. paratuberculosis antigens. Purified antigen 20 was identified as alkyl hydroperoxide reductase C (AhpC) while the N-terminal part of purified antigen 17 showed 80% homology with alkyl hydroperoxide reductase D (AhpD) of Mycobacterium tuberculosis. AhpC had a nonreduced mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis corresponding to a molecular mass of 45 kDa and is probably a homodimer linked with disulfide bridges in its native form. AhpD had a mobility corresponding to 19 kDa. Monospecific rabbit antiserum against AhpC and AhpD reacted with 9 strains of M. avium subsp. paratuberculosis but not with 20 other mycobacterial strains except for a Mycobacterium gordonae strain, against which a weak cross-reactive band was produced. Goats experimentally infected with M. avium subsp. paratuberculosis had strong gamma interferon (IFN-gamma) responses toward both AhpC and AhpD, and they also had antibodies against AhpC. The ability of AhpC and AhpD to induce IFN-gamma production shows that these proteins potentially could be used in future vaccines or in diagnostic assays. These results further show that AhpC and AhpD are immunologically important proteins which are constitutively and highly expressed in M. avium subsp. paratuberculosis without the bacteria being submitted to oxidative stress and that the specificities of antigens can be a matter of different levels of protein expression in various species as well as distinct structural differences.
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