ArticleAntimicrobial agents and chemotherapy2000
Influence of shaking on antifungal susceptibility testing of Cryptococcus neoformans: a comparison of the NCCLS standard M27A medium, buffered yeast nitrogen base, and RPMI-2% glucose.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- N-terminal lipidation enhances membrane interaction and antifungal activity of a Trematocine-derived decapeptide.Applied microbiology and biotechnology · 2026Article
- Factors influencing susceptibility testing of antifungal drugs: a critical review of document M27-A4 from the Clinical and Laboratory Standards Institute (CLSI).Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Review
- Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds.Journal of visualized experiments : JoVE · 2018Article
- Comparison of different in vitro tests to detect Cryptococcus neoformans not susceptible to amphotericin B.Mycopathologia · 2015Article
- Process analysis of variables for standardization of antifungal susceptibility testing of nonfermentative yeasts.Antimicrobial agents and chemotherapy · 2011Article
- Comparison of the Vitek 2 antifungal susceptibility system with the clinical and laboratory standards institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) Broth Microdilution Reference Methods and with the Sensititre YeastOne and Etest techniques for in vitro detection of antifungal resistance in yeast isolates.Journal of clinical microbiology · 2010Article
- Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
- Reliability of the WIDERYST susceptibility testing system for detection of in vitro antifungal resistance in yeasts.Antimicrobial agents and chemotherapy · 2008Article
- Subcutaneous cryptococcosis due to Cryptococcus diffluens in a patient with sporotrichoid lesions case report, features of the case isolate and in vitro antifungal susceptibilities.Medical mycology · 2007Article
- Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi.Antimicrobial agents and chemotherapy · 2006Article
- Susceptibility patterns and molecular identification of Trichosporon species.Antimicrobial agents and chemotherapy · 2005Article
- In vitro activities of ravuconazole and four other antifungal agents against fluconazole-resistant or -susceptible clinical yeast isolates.Antimicrobial agents and chemotherapy · 2004Article
- Clinical case of endocarditis due to Trichosporon inkin and antifungal susceptibility profile of the organism.Journal of clinical microbiology · 2004Article
- Genotyping and antifungal susceptibility profile of Dipodascus capitatus isolates causing disseminated infection in seven hematological patients of a tertiary hospital.Journal of clinical microbiology · 2004Article
- G484S amino acid substitution in lanosterol 14-alpha demethylase (ERG11) is related to fluconazole resistance in a recurrent Cryptococcus neoformans clinical isolate.Antimicrobial agents and chemotherapy · 2003Article
- Trends in antifungal drug susceptibility of Cryptococcus neoformans isolates in the United States: 1992 to 1994 and 1996 to 1998.Antimicrobial agents and chemotherapy · 2001Article
- Antifungal susceptibility testing: practical aspects and current challenges.Clinical microbiology reviews · 2001Review
- Influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of Candida spp.Journal of clinical microbiology · 2001Article
- Analysis of growth characteristics of filamentous fungi in different nutrient media.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococcus neoformans is a nonfermentative yeast that requires oxygen for growth. The shaking of culture media achieves good oxygenation, promoting the growth of cryptococci. In this study, three test media (RPMI 1640, RPMI 1640-2% glucose, and buffered yeast nitrogen base ¿BYNB) recommended in the National Committee for Clinical Laboratory Standards M27A standard were examined. Growth abilities and minimum inhibitory concentrations (MICs) in microplates incubated at 35 degrees C for 48 h were determined. The results indicated that shaking and an inoculum size of 10(5) CFU/ml yielded optimal growth of this yeast. Compared to RPMI 1640, supplementation of RPMI 1640 with 2% glucose did not significantly improve growth of C. neoformans and resulted in an 8.7-h delay of exponential growth. Cryptococcal growth in RPMI 1640 at 24 h was notably better than that in RPMI-2% glucose, although by 48 h the growths were comparable. The MIC range of amphotericin B observed for the C. neoformans strains grown in RPMI 1640 with or without glucose was too narrow to allow the separation of susceptible and resistant strains based on clinical outcome. The widest ranges of MICs of flucytosine and fluconazole were obtained with BYNB. This work demonstrates the need for a new antifungal susceptibility test for C. neoformans.
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