ArticleAntimicrobial agents and chemotherapy2000
Comparison of three methods of determining MICs for filamentous fungi using different end point criteria and incubation periods.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- An automated workflow for fungal cell counting, cell size and data analysis: enhancing throughput and accuracy with the cell analysis and counting tool using ilastik software (caactus).BMC microbiology · 2026Article
- Azole and Amphotericin B MIC Values againstAntimicrobial agents and chemotherapy · 2020Article
- Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) fromAntibiotics (Basel, Switzerland) · 2020Article
- Fungal biomass is a key factor affecting polymorphonuclear leucocyte-induced hyphal damage of filamentous fungi.Mycoses · 2010Article
- Establishing a method of inoculum preparation for susceptibility testing of Trichophyton rubrum and Trichophyton mentagrophytes.Journal of clinical microbiology · 2006Article
- Evaluation of broth microdilution antifungal susceptibility testing conditions for Trichophyton rubrum.Journal of clinical microbiology · 2005Article
- Testing of the in vitro susceptibilities of Madurella mycetomatis to six antifungal agents by using the Sensititre system in comparison with a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay and a modified NCCLS method.Antimicrobial agents and chemotherapy · 2005Article
- Screening for microtubule-disrupting antifungal agents by using a mitotic-arrest mutant of Aspergillus nidulans and novel action of phenylalanine derivatives accompanying tubulin loss.Antimicrobial agents and chemotherapy · 2004Article
- Collaborative evaluation of optimal antifungal susceptibility testing conditions for dermatophytes.Journal of clinical microbiology · 2002Article
- Comparison of spectrophotometric and visual readings of NCCLS method and evaluation of a colorimetric method based on reduction of a soluble tetrazolium salt, 2,3-bis [2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide], for antifungal susceptibility testing of Aspergillus species.Journal of clinical microbiology · 2001Article
- Analysis of growth characteristics of filamentous fungi in different nutrient media.Journal of clinical microbiology · 2001Article
- Influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of Candida spp.Journal of clinical microbiology · 2001Article
- Novel hydroxamates potentiatedJournal of natural science, biology, and medicineArticle
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6 authors.
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Abstract
Three different methods were used to determine the in vitro activities of amphotericin B, ketoconazole, itraconazole, and flucytosine against 30 isolates of different genera of filamentous fungi. MICs were determined visually, with or without agitation, and spectrophotometrically by using a broth microdilution method. For amphotericin B, there was one end point reading criterion (the minimum concentration of antifungal that inhibited 100% of growth), but for azoles and flucytosine there were two (the minimum concentrations that inhibited 50 and 75% of fungal growth, respectively) after 48 and 72 h of incubation. All tests were performed in triplicate. An intraclass correlation coefficient (ICC) was used to evaluate the reproducibility of each of the methods and the correlation among them. The reproducibility of the three methods was very high (ICC of 0.808 to 0.992), particularly in the case of azoles and flucytosine. In general, the degree of reproducibility was highest for azoles and amphotericin B after 72 h of incubation and for flucytosine after 48 h of incubation. The degree of correlation among the three methods was very high (ICC of >0.98) with all of the antifungals under all the conditions tested. The end point reading criteria and the time of incubation affected neither the reproducibility of the methods nor their correlation, and their effect on MICs was statistically significant.
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