ArticleAntimicrobial agents and chemotherapy2000
Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 167 papers, 1 of them a synthesis that pooled it.
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Who cites it
167 citing papers in PubMed, 1 synthesis or guideline pooled it, 423 citations in OpenAlex.
- Pooled it
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- Companion animals as potentially important reservoir of methicillin-resistant and virulent strains of coagulase-positive Staphylococcus spp.BMC veterinary research · 2026Article
- One Health Genomic Analysis of Methicillin-Resistant Staphylococcus spp. From Humans, Cats, and Dogs in a Veterinary Hospital in Central Chile.Zoonoses and public health · 2026Article
- Perinatal Occurrence and Epidemiological Significance ofAnimals : an open access journal from MDPI · 2026Article
- Molecular Profiling of Foodborne Pathogens in Ready-to-Eat Foods, Al-Madinah Al-Munawarah, Saudi Arabia.Biology · 2026Article
- Clinical and genomic characterization ofFrontiers in microbiology · 2026Article
- Characterization of Staphylococci colonizing healthy equine skin: antibiotic resistance, virulence factors, and biofilm formation.Veterinary research communications · 2025Article
- High colonization by multidrug-resistant and virulent Staphylococcus aureus genotypes among critically ill patients in the COVID-19 pandemic in a Brazilian hospital.BMC microbiology · 2025Article
- Genotypic and Technological Characterization of Lactic Acid Bacteria and Coagulase-Negative Staphylococci Isolated from Sucuk: A Preliminary Screening of Potential Starter Cultures.Foods (Basel, Switzerland) · 2025Article
- Comparative Analysis of Phenotypic and Genotypic Antibiotic Susceptibility ofAntibiotics (Basel, Switzerland) · 2025Article
- Genomic analysis reveals two dominant strains ofApplied and environmental microbiology · 2025Article
- Antibacterial Activity of Clove Essential Oil (Pathogens (Basel, Switzerland) · 2025Article
- Validation of a modified rapid test to detect the cefazolin inoculum effect in methicillin-susceptible Staphylococcus aureus from bloodstream infections in hospitals from North and Latin America.The Journal of antimicrobial chemotherapy · 2025Article
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- HarnessingNanomaterials (Basel, Switzerland) · 2025Article
- Prevalence, antimicrobial resistance and biofilm forming ability ofFrontiers in veterinary science · 2025Article
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- Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications.BMC research notes · 2024Article
107 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Clinical isolates of Staphylococcus aureus (a total of 206) and S. epidermidis (a total of 188) from various countries were tested with multiplex PCR assays to detect clinically relevant antibiotic resistance genes associated with staphylococci. The targeted genes are implicated in resistance to oxacillin (mecA), gentamicin ¿aac(6')-aph(2"), and erythromycin (ermA, ermB, ermC, and msrA). We found a nearly perfect correlation between genotypic and phenotypic analysis for most of these 394 strains, showing the following correlations: 98% for oxacillin resistance, 100% for gentamicin resistance, and 98.5% for erythromycin resistance. The discrepant results were (i) eight strains found to be positive by PCR for mecA or ermC but susceptible to the corresponding antibiotic based on disk diffusion and (ii) six strains of S. aureus found to be negative by PCR for mecA or for the four erythromycin resistance genes targeted but resistant to the corresponding antibiotic. In order to demonstrate in vitro that the eight susceptible strains harboring the resistance gene may become resistant, we subcultured the susceptible strains on media with increasing gradients of the antibiotic. We were able to select cells demonstrating a resistant phenotype for all of these eight strains carrying the resistance gene based on disk diffusion and MIC determinations. The four oxacillin-resistant strains negative for mecA were PCR positive for blaZ and had the phenotype of beta-lactamase hyperproducers, which could explain their borderline oxacillin resistance phenotype. The erythromycin resistance for the two strains found to be negative by PCR is probably associated with a novel mechanism. This study reiterates the usefulness of DNA-based assays for the detection of antibiotic resistance genes associated with staphylococcal infections.
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