ArticleProceedings of the National Academy of Sciences of the United States of America2000
An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 179 citations in OpenAlex.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- Jan and mini-Jan, a model system for potato functional genomics.Plant biotechnology journal · 2025Article
- Improving transformation and regeneration efficiency in medicinal plants: insights from other recalcitrant species.Journal of experimental botany · 2025Review
- Jan and mini-Jan, a model system for potato functional genomics.bioRxiv : the preprint server for biology · 2024Article
- Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation.Nature communications · 2022Article
- Increased Agrobacterium-mediated transformation of Saccharomyces cerevisiae after deletion of the yeast ADA2 gene.Letters in applied microbiology · 2022Article
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Co-located quantitative trait loci mediate resistance to Agrobacterium tumefaciens, Phytophthora cinnamomi, and P. pini in Juglans microcarpa × J. regia hybrids.Horticulture research · 2021Article
- Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase θ.The New phytologist · 2021Article
- F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation.International journal of molecular sciences · 2020Article
- Review
- Article
- Single Molecule Imaging of T-DNA Intermediates FollowingInternational journal of molecular sciences · 2019Article
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamiana.Plant biotechnology journal · 2018Article
- Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation.International journal of molecular sciences · 2018Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Mitochondrial Porin Isoform AtVDAC1 Regulates the Competence of Arabidopsis thaliana to Agrobacterium-Mediated Genetic Transformation.Molecules and cells · 2016Article
- Transcriptomic Profiling Analysis of Arabidopsis thaliana Treated with Exogenous Myo-Inositol.PloS one · 2016Article
3 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens genetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, the T-DNA, to the plant and integrating the T-DNA into the plant genome. Little is known about the T-DNA integration process, and no plant genes involved in integration have yet been identified. We characterized an Arabidopsis mutant generated by T-DNA insertional mutagenesis, rat5, that is resistant to Agrobacterium root transformation. rat5 contains two copies of T-DNA integrated as a tandem direct repeat into the 3' untranslated region of a histone H2A gene, upstream of the polyadenylation signal sequence. Transient and stable beta-glucuronidase expression data and assessment of the amount of T-DNA integrated into the genomes of wild-type and rat5 Arabidopsis plants indicated that the rat5 mutant is deficient in T-DNA integration. We complemented the rat5 mutation by expressing the RAT5 histone H2A gene in the mutant plant. Overexpression of RAT5 in wild-type plants increased Agrobacterium transformation efficiency. Furthermore, transient expression of a RAT5 gene from the incoming T-DNA was sufficient to complement the rat5 mutant and to increase the transformation efficiency of wild-type Arabidopsis plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.