ArticleProceedings of the National Academy of Sciences of the United States of America2000
Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Distinct subunit contributions to the activation of M-type potassium channels by PI(4,5)P2.The Journal of general physiology · 2012Article
- Subunit counting in membrane-bound proteins.Nature methods · 2007Article
- Locking CNGA1 channels in the open and closed state.Biophysical journal · 2006Article
- Stabilization of the activity of ATP-sensitive potassium channels by ion pairs formed between adjacent Kir6.2 subunits.The Journal of general physiology · 2003Article
- Selective heteromeric assembly of cyclic nucleotide-gated channels.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Studies of NMDA receptor function and stoichiometry with truncated and tandem subunits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Movement of the C-helix during the gating of cyclic nucleotide-gated channels.Biophysical journal · 2002Article
- Article
- Gating of heteromeric retinal rod channels by cyclic AMP: role of the C-terminal and pore domains.Biophysical journal · 2002Article
- Mechanism of calcium/calmodulin inhibition of rod cyclic nucleotide-gated channels.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular architecture of a retinal cGMP-gated channel: the arrangement of the cytoplasmic domains.The EMBO journal · 2002Article
- Efficient coupling of ligand binding to channel opening by the binding domain of a modulatory (beta) subunit of the olfactory cyclic nucleotide-gated channel.The Journal of general physiology · 2001Article
- Fraction of the dark current carried by Ca(2+) through cGMP-gated ion channels of intact rod and cone photoreceptors.The Journal of general physiology · 2000Article
- Direct association of ligand-binding and pore domains in homo- and heterotetrameric inositol 1,4,5-trisphosphate receptors.The EMBO journal · 2000Article
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Authors and funding
3 authors.
Funding
Abstract
Retinal rod cyclic nucleotide-gated channels are composed of alpha and beta subunits. We have explored possible subunit arrangements by expressing tandemly linked dimers of both subunits and examining their responses to three different modulating agents. Channels formed from either alpha-beta or beta-alpha heterodimers had functional properties similar to those formed from coexpressed alpha and beta monomers and to native channels. These results point to an alpha-beta-alpha-beta arrangement. To ensure that heterodimers had not flipped around, we coexpressed alpha-alpha dimers with an excess of either beta monomers or beta-beta dimers. Our data indicate that heteromultimers do not form efficiently in an alpha-alpha-beta-beta arrangement. Thus, we propose that native rod cyclic nucleotide-gated channels are arranged with like subunits diagonally opposed: alpha-beta-alpha-beta.
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