ArticleProceedings of the National Academy of Sciences of the United States of America2000
Genetic analysis of a bacterial genetic exchange element: the gene transfer agent of Rhodobacter capsulatus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed, 187 citations in OpenAlex.
- Perturbations shift the composition of bacterial DNA carried by virus-like particles in the murine gut microbiome.bioRxiv : the preprint server for biology · 2026Article
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- Naturally competent bacteria and their genetic parasites-a battle for control over horizontal gene transfer?FEMS microbiology reviews · 2025Review
- Metagenomic Insights into Candidatus Scalindua in a Long-term Cultivated Marine Anammox Consortium: The Important Role of Tetrahydrofolate-mediated Carbon Fixation.Microbes and environments · 2025Article
- New evidence supports the prophage origin of RcGTA.Applied and environmental microbiology · 2024Article
- Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination.Nature communications · 2024Article
- Co-evolution of gene transfer agents and their alphaproteobacterial hosts.Journal of bacteriology · 2024Article
- Bacterial community and genome analysis of cytoplasmic incompatibility-inducingFrontiers in microbiology · 2024Article
- Structure and proposed DNA delivery mechanism of a marine roseophage.Nature communications · 2023Article
- Selection for Translational Efficiency in Genes Associated with Alphaproteobacterial Gene Transfer Agents.mSystems · 2022Article
- Expansion of Known Rhodobacter capsulatus Bacteriophage Diversity with 24 Additional Genomes.Microbiology resource announcements · 2022Article
- Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.PHAGE (New Rochelle, N.Y.) · 2022Article
- DNA Gyrase Inhibitors Increase the Frequency of Bacteriophage-like RcGTA-Mediated Gene Transfer inGenes · 2022Article
- Virus-derived gene transfer agents benefit host cells by providing templates for DNA repair.PLoS biology · 2022Article
- Shared properties of gene transfer agent and core genes revealed by comparative genomics of Alphaproteobacteria.Microbial genomics · 2022Article
- Loss of the Rhodobacter capsulatus Serine Acetyl Transferase Gene,Applied and environmental microbiology · 2022Article
- The archetypal gene transfer agent RcGTA is regulated via direct interaction with the enigmatic RNA polymerase omega subunit.Cell reports · 2022Article
- Widespread phages of endosymbionts: Phage WO genomics and the proposed taxonomic classification of Symbioviridae.PLoS genetics · 2022Article
- Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP inMicroorganisms · 2022Article
- Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An unusual system of genetic exchange exists in the purple nonsulfur bacterium Rhodobacter capsulatus. DNA transmission is mediated by a small bacteriophage-like particle called the gene transfer agent (GTA) that transfers random 4.5-kb segments of the producing cell's genome to recipient cells, where allelic replacement occurs. This paper presents the results of gene cloning, analysis, and mutagenesis experiments that show that GTA resembles a defective prophage related to bacteriophages from diverse genera of bacteria, which has been adopted by R. capsulatus for genetic exchange. A pair of cellular proteins, CckA and CtrA, appear to constitute part of a sensor kinase/response regulator signaling pathway that is required for expression of GTA structural genes. This signaling pathway controls growth-phase-dependent regulation of GTA gene messages, yielding maximal gene expression in the stationary phase. We suggest that GTA is an ancient prophage remnant that has evolved in concert with the bacterial genome, resulting in a genetic exchange process controlled by the bacterial cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.