ArticleProceedings of the National Academy of Sciences of the United States of America2000
Calnuc, an EF-hand Ca(2+) binding protein, specifically interacts with the C-terminal alpha5-helix of G(alpha)i3.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
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- Aneuploid CTC and CEC.Diagnostics (Basel, Switzerland) · 2018Review
- Comprehensive in situ co-detection of aneuploid circulating endothelial and tumor cells.Scientific reports · 2017Article
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- Identification of steroid-sensitive gene-1/Ccdc80 as a JAK2-binding protein.Molecular endocrinology (Baltimore, Md.) · 2013Article
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- Secreted nucleobindin-2 inhibits 3T3-L1 adipocyte differentiation.Protein and peptide letters · 2012Article
- G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs.The Journal of biological chemistry · 2011Article
- Nesfatin-1: a novel inhibitory regulator of food intake and body weight.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2011Review
- Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.The Journal of biological chemistry · 2010Article
- Nesfatin-1--role as possible new potent regulator of food intake.Regulatory peptides · 2010Review
- Molecular characterisation and expression analysis of SEREX-defined antigen NUCB2 in gastric epithelium, gastritis and gastric cancer.European journal of histochemistry : EJH · 2009Review
- Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells.Molecular neurodegeneration · 2009Article
- Interaction of penicillin-binding protein 2 with soluble lytic transglycosylase B1 in Pseudomonas aeruginosa.Journal of bacteriology · 2008Article
- Nucleobindin co-localizes and associates with cyclooxygenase (COX)-2 in human neutrophils.PloS one · 2008Article
- Modulation of nucleobindin-1 and nucleobindin-2 by caspases.FEBS letters · 2008Article
- Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components.The Journal of biological chemistry · 2005Article
- Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Calnuc (nucleobindin) was previously shown to be present both in the cytosol and in the Golgi and to be the major Golgi Ca(2+) binding protein. In this study we verified the existence of the cytosolic pool of calnuc and investigated its interaction with G(alpha)i3. Cytosolic calnuc was released by mild digitonin permeabilization. In pulse-chase experiments, the two pools of calnuc had different mobilities, suggesting different posttranslational modifications. That calnuc interacts with G(alpha)i3 in vivo was verified by the finding that G(alpha)i3 could be crosslinked intracellularly to calnuc and co-immunoprecipitated from NIH 3T3 cells stably overexpressing either activated (Q204L) or inactivated (G203A) G(alpha)i3. Binding was Ca(2+) and Mg(2+)-dependent. Calnuc and G(alpha)i3-GFP codistributed primarily in the Golgi region. By yeast two-hybrid analysis, the binding site on G(alpha)i3 for calnuc was mapped to the C-terminal region because removal of the last 12 amino acids (but not 11) abolished the interaction. Peptide competition indicated that calnuc, with its coiled-coil domain constituted by the two EF-hands, binds to G(alpha)i3's C-terminal alpha5-helix. These results demonstrate that calnuc may play an important role in G protein- and Ca(2+)-regulated signal transduction events.
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