ArticleThe Journal of physiology2000
Inactivation of P2X2 purinoceptors by divalent cations.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Analysis of Spatial and Temporal Distribution of Purinergic P2 Receptors in the Mouse Hippocampus.International journal of molecular sciences · 2021Article
- Article
- Localization of the gate and selectivity filter of the full-length P2X7 receptor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Role of domain calcium in purinergic P2X2 receptor channel desensitization.American journal of physiology. Cell physiology · 2015Article
- Regulation of ATP-gated P2X channels: from redox signaling to interactions with other proteins.Antioxidants & redox signaling · 2014Review
- Heteromeric assembly of P2X subunits.Frontiers in cellular neuroscience · 2013Review
- Desensitization properties of P2X3 receptors shaping pain signaling.Frontiers in cellular neuroscience · 2013Review
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- Gating properties of the P2X2a and P2X2b receptor channels: experiments and mathematical modeling.The Journal of general physiology · 2012Article
- Receptor and channel heteromers as pain targets.Pharmaceuticals (Basel, Switzerland) · 2012Article
- Communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (P2) and glutamatergic receptors.PloS one · 2012Article
- Activation and regulation of purinergic P2X receptor channels.Pharmacological reviews · 2011Review
- Expression and functional characterization of P2X receptors in mouse alveolar macrophages.Pflugers Archiv : European journal of physiology · 2011Article
- Aminoglycoside block of P2X2 receptors heterologously expressed in Xenopus laevis oocytes.Purinergic signalling · 2010Article
- Voltage- and [ATP]-dependent gating of the P2X(2) ATP receptor channel.The Journal of general physiology · 2009Article
- Activation kinetics of single P2X receptors.Purinergic signalling · 2007Article
- Responses of rat P2X2 receptors to ultrashort pulses of ATP provide insights into ATP binding and channel gating.The Journal of general physiology · 2007Article
- Regulation of the desensitization and ion selectivity of ATP-gated P2X2 channels by phosphoinositides.The Journal of physiology · 2006Article
- Biophysics of P2X receptors.Pflugers Archiv : European journal of physiology · 2006Review
- Bimodal action of protons on ATP currents of rat PC12 cells.The Journal of general physiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. P2X2 channels are activated by extracellular ATP. Despite being commonly described as non-desensitizing, P2X2 receptors do desensitize or inactivate. In the unspliced, 472 amino acid isoform of the P2X2 receptor, inactivation required membrane disruption and the presence of extracellular Ca2+. 2. The ability to inactivate whole-cell currents developed slowly after breaking in. In contrast, currents from excised patches exhibited rapid (approximately 100 ms) inactivation with a dependence on extracellular Ca2+, ATP and voltage. 3. The inactivation rate increased with the fourth power of [Ca2+] suggesting that the functional channel may be a tetramer. Ca2+ had both a higher affinity and a larger Hill coefficient for inactivation than Mg2+, Ba2+ or Mn2+. Trivalent cations at concentrations up to the solubility product of ATP had no effect. The change in apparent co-operativity with ionic species suggests the presence of experimentally unresolved ligand-insensitive kinetic steps. 4. Based on the weak voltage dependence of inactivation and the lack of effect of intracellular Ca2+ buffers, the Ca2+-binding sites are probably located near the extracellular surface of the membrane. 5. The recovery from inactivation was slow, with a time constant of approximately 7 min. 6. Ca2+-sensitive inactivation only appeared when the membrane was disrupted in some manner. Treatment with actin and microtubule reagents did not induce inactivation, suggesting that an intact cytoskeleton is not necessary. 7. Inactivation rates observed in different patch configurations suggest that the induction of Ca2+-dependent inactivation was due to the loss of a diffusible cofactor located in the membrane or the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.