ArticleNucleic acids research2000
cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 114 citations in OpenAlex.
- Mobility of mPing and its associated elements is regulated by both internal and terminal sequences.Mobile DNA · 2023Article
- Development ofPlant direct · 2021Article
- Global survey of mobile DNA horizontal transfer in arthropods reveals Lepidoptera as a prime hotspot.PLoS genetics · 2019Article
- Experimental evolution reveals hyperparasitic interactions among transposable elements.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A transposon-based tool for transformation and mutagenesis in trypanosomatid protozoa.Methods in molecular biology (Clifton, N.J.) · 2015Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart.The Journal of biological chemistry · 2014Article
- Biochemical characterization and comparison of two closely related active mariner transposases.Biochemistry · 2014Article
- DNA transposon-based gene vehicles - scenes from an evolutionary drive.Journal of biomedical science · 2013Review
- A rice Stowaway MITE for gene transfer in yeast.PloS one · 2013Article
- Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification.BMC genomics · 2012Article
- Article
- Genetic manipulation of Methanosarcina spp.Frontiers in microbiology · 2012Article
- Nuclear importation of Mariner transposases among eukaryotes: motif requirements and homo-protein interactions.PloS one · 2011Article
- Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Review
- Article
- Review
- Target site selection by the mariner-like element, Mos1.Genetica · 2010Article
- DNA transposons: nature and applications in genomics.Current genomics · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mos1 and other mariner / Tc1 transposons move horizon-tally during evolution, and when transplanted into heterologous species can transpose in organisms ranging from prokaryotes to protozoans and vertebrates. To further develop the Drosophila Mos1 mariner system as a genetic tool and to probe mechanisms affecting the regulation of transposition activity, we developed an in vitro system for Mos1 transposition using purified transposase and selectable Mos1 derivatives. Transposition frequencies of nearly 10(-3)/target DNA molecule were obtained, and insertions occurred at TA dinucleotides with little other sequence specificity. Mos1 elements containing only the 28 bp terminal inverted repeats were inactive in vitro, while elements containing a few additional internal bases were fully active, establishing the minimal cis -acting requirements for transposition. With increasing transposase the transposition frequency increased to a plateau value, in contrast to the predictions of the protein over-expression inhibition model and to that found recently with a reconstructed Himar1 transposase. This difference between the 'natural' Mos1 and 'reconstructed' Himar1 transposases suggests an evolutionary path for down-regulation of mariner transposition following its introduction into a naïve population. The establishment of the cis and trans requirements for optimal mariner transposition in vitro provides key data for the creation of vectors for in vitro mutagenesis, and will facilitate the development of in vivo systems for mariner transposition.
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