ArticleNucleic acids research2000
The efficiency of Escherichia coli selenocysteine insertion is influenced by the immediate downstream nucleotide.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- deep-Sep: a deep learning-based method for fast and accurate prediction of selenoprotein genes in bacteria.mSystems · 2025Article
- Expanding the chemical diversity of M13 bacteriophage.Frontiers in microbiology · 2022Review
- Selenium utilization in thioredoxin and catalytic advantage provided by selenocysteine.Biochemical and biophysical research communications · 2015Article
- Multivalent site-specific phage modification enhances the binding affinity of receptor ligands.Bioconjugate chemistry · 2015Article
- Bacteriophage vehicles for phage display: biology, mechanism, and application.Current microbiology · 2014Review
- Wobble decoding by the Escherichia coli selenocysteine insertion machinery.Nucleic acids research · 2013Article
- Tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase.Molecular microbiology · 2011Article
- In-depth genetic analysis of Clostridium difficile PCR-ribotype 027 strains reveals high genome fluidity including point mutations and inversions.Gut microbes · 2010Article
- Phage-encoded combinatorial chemical libraries based on bicyclic peptides.Nature chemical biology · 2009Article
- Semisynthesis and characterization of mammalian thioredoxin reductase.Biochemistry · 2006Article
- Selenocysteine insertion directed by the 3'-UTR SECIS element in Escherichia coli.Nucleic acids research · 2005Article
- Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases.Molecular biology of the cell · 2004Article
- Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants.Nucleic acids research · 2003Article
- A dynamic competition between release factor 2 and the tRNA(Sec) decoding UGA at the recoding site of Escherichia coli formate dehydrogenase H.The EMBO journal · 2001Article
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2 authors.
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Abstract
Selenocysteine (Sec) incorporation requires the TGA opal codon and a downstream Sec insertion sequence (SECIS), which can be partially randomized and cloned into M13 pIII fusion constructs for phage display. This combinatorial approach provides a convenient non-radioactive assay that couples phage production to opal suppression. Two SECIS libraries were prepared, with the immediate downstream nucleotide either randomized (TGAN) or fixed as thymidine (TGAT). The TGAN library resulted in a majority of clones with a downstream purine and selenium-independent phage production, implicating the endo-genous tryptophan-inserting opal suppression pathway. Although the addition of sodium selenite to the growth medium did not affect phage production, it did increase the level of Sec insertion, as shown by the chemical reactivity of the resulting phage. The TGAT phage library yielded clones with strictly selenium-dependent phage production and reactivity consistent with the presence of Sec. These clones were prone to spontaneous mutation upon further propagation, however, resulting in loss of the selenium-dependent phenotype. We conclude that the immediate downstream nucleotide determines whether the endogenous opal suppression pathway competes with co-translational Sec insertion.
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