ArticleMolecular biology of the cell2000
Toxofilin, a novel actin-binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Highly divergent apicomplexan cytoskeletons provide additional models for actin biology.The FEBS journal · 2025Review
- A Role for Basigin in Toxoplasma gondii Infection.Infection and immunity · 2022Article
- A conserved coccidian gene is involved in Toxoplasma sensitivity to the anti-apicomplexan compound, tartrolon E.International journal for parasitology. Drugs and drug resistance · 2020Article
- Article
- Study on Circulating Antigens in Serum of Mice With Experimental Acute Toxoplasmosis.Frontiers in microbiology · 2020Article
- Translocation of effector proteins into host cells by Toxoplasma gondii.Current opinion in microbiology · 2019Review
- Article
- Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.Journal of cell science · 2012Article
- Malaria parasite actin polymerization and filament structure.The Journal of biological chemistry · 2010Article
- Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.Nature methods · 2010Article
- A highly sensitive FRET-based approach reveals secretion of the actin-binding protein toxofilin during Toxoplasma gondii infection.Cellular microbiology · 2010Article
- Communication between Toxoplasma gondii and its host: impact on parasite growth, development, immune evasion, and virulence.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2009Review
- Expression quantitative trait locus mapping of toxoplasma genes reveals multiple mechanisms for strain-specific differences in gene expression.Eukaryotic cell · 2008Article
- Rhoptries: an arsenal of secreted virulence factors.Current opinion in microbiology · 2007Review
- ARL4D recruits cytohesin-2/ARNO to modulate actin remodeling.Molecular biology of the cell · 2007Article
- Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimer.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The actin multigene family of Paramecium tetraurelia.BMC genomics · 2007Article
- The toxofilin-actin-PP2C complex of Toxoplasma: identification of interacting domains.The Biochemical journal · 2007Article
- Cytoskeletal components of an invasion machine--the apical complex of Toxoplasma gondii.PLoS pathogens · 2006Article
- Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles.PLoS pathogens · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxoplasma gondii relies on its actin cytoskeleton to glide and enter its host cell. However, T. gondii tachyzoites are known to display a strikingly low amount of actin filaments, which suggests that sequestration of actin monomers could play a key role in parasite actin dynamics. We isolated a 27-kDa tachyzoite protein on the basis of its ability to bind muscle G-actin and demonstrated that it interacts with parasite G-actin. Cloning and sequence analysis of the gene coding for this protein, which we named Toxofilin, showed that it is a novel actin-binding protein. In in vitro assays, Toxofilin not only bound to G-actin and inhibited actin polymerization as an actin-sequestering protein but also slowed down F-actin disassembly through a filament end capping activity. In addition, when green fluorescent protein-tagged Toxofilin was overexpressed in mammalian nonmuscle cells, the dynamics of actin stress fibers was drastically impaired, whereas green fluorescent protein-Toxofilin copurified with G-actin. Finally, in motile parasites, during gliding or host cell entry, Toxofilin was localized in the entire cytoplasm, including the rear end of the parasite, whereas in intracellular tachyzoites, especially before they exit from the parasitophorous vacuole of their host cell, Toxofilin was found to be restricted to the apical end.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.