ArticleMolecular biology of the cell2000
Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Review
- Rac1-PAK1 regulation of Rab11 cycling promotes junction destabilization.The Journal of cell biology · 2021Article
- CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2.PLoS genetics · 2012Article
- Article
- Concordance and interaction of guanine nucleotide dissociation inhibitor (RhoGDI) with RhoA in oogenesis and early development of the sea urchin.Development, growth & differentiation · 2011Article
- Roles of small GTPase Rac1 in the regulation of actin cytoskeleton during dengue virus infection.PLoS neglected tropical diseases · 2010Article
- Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN.Nature cell biology · 2010Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Cdc42 and the phosphatidylinositol 3-kinase-Akt pathway are essential for PspC-mediated internalization of pneumococci by respiratory epithelial cells.The Journal of biological chemistry · 2009Article
- C. elegans CARMIL negatively regulates UNC-73/Trio function during neuronal development.Development (Cambridge, England) · 2009Article
- Differentiation restricted endocytosis of cell penetrating peptides in MDCK cells corresponds with activities of Rho-GTPases.Pharmaceutical research · 2007Article
- Rho GTPases and leucocyte-induced endothelial remodelling.The Biochemical journal · 2005Review
- Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation.Molecular biology of the cell · 2003Article
- Myelin biogenesis: vesicle transport in oligodendrocytes.Neurochemical research · 2002Review
- Regulation of protein transport from the Golgi complex to the endoplasmic reticulum by CDC42 and N-WASP.Molecular biology of the cell · 2002Article
- Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 2001Article
- Interaction with mLin-7 alters the targeting of endocytosed transmembrane proteins in mammalian epithelial cells.Molecular biology of the cell · 2001Article
- Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding.The Journal of cell biology · 2001Article
- Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Madin-Darby canine kidney (MDCK) cells expressing constitutively active Rac1 (Rac1V12) accumulate a large central aggregate of membranes beneath the apical membrane that contains filamentous actin, Rac1V12, rab11, and the resident apical membrane protein GP-135. To examine the roles of Rac1 in membrane traffic and the formation of this aggregate, we analyzed endocytic and biosynthetic trafficking pathways in MDCK cells expressing Rac1V12 and dominant inactive Rac1 (Rac1N17). Rac1V12 expression decreased the rates of apical and basolateral endocytosis, whereas Rac1N17 expression increased those rates from both membrane domains. Basolateral-to-apical transcytosis of immunoglobulin A (IgA) (a ligand for the polymeric immunoglobulin receptor [pIgR]), apical recycling of pIgR-IgA, and accumulation of newly synthesized GP-135 at the apical plasma membrane were all decreased in cells expressing Rac1V12. These effects of Rac1V12 on trafficking pathways to the apical membrane were the result of the delivery and trapping of these proteins in the central aggregate. In contrast to abnormalities in apical trafficking events, basolateral recycling of transferrin, degradation of EGF internalized from the basolateral membrane, and delivery of newly synthesized pIgR from the Golgi to the basolateral membrane were all relatively unaffected by Rac1V12 expression. Rac1N17 expression had little or no effect on these postendocytic or biosynthetic trafficking pathways. These results show that in polarized MDCK cells activated Rac1 may regulate the rate of endocytosis from both membrane domains and that expression of dominant active Rac1V12 specifically alters postendocytic and biosynthetic membrane traffic directed to the apical, but not the basolateral, membrane.
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