ArticleMolecular biology of the cell2000
The N terminus of the transmembrane protein BP180 interacts with the N-terminal domain of BP230, thereby mediating keratin cytoskeleton anchorage to the cell surface at the site of the hemidesmosome.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 115 citations in OpenAlex.
- Autoimmune bullous diseases: pathogenesis and clinical management.Molecular biomedicine · 2025Review
- Native Autoantigen Complex Detects Pemphigoid Autoantibodies.JID innovations : skin science from molecules to population health · 2023Article
- Junctional epithelium and hemidesmosomes: Tape and rivets for solving the "percutaneous device dilemma" in dental and other permanent implants.Bioactive materials · 2022Review
- Collagen XVII deficiency alters epidermal patterning.Laboratory investigation; a journal of technical methods and pathology · 2022Article
- Bullous pemphigoid: An immune disorder related to aging (Review).Experimental and therapeutic medicine · 2022Review
- Non-Cell-Autonomous Activity of the Hemidesmosomal Protein BP180/Collagen XVII in Granulopoiesis in Humanized NC16A Mice.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- The Role of Collagen XVII in Cancer: Squamous Cell Carcinoma and Beyond.Frontiers in oncology · 2020Review
- The spectraplakin Dystonin antagonizes YAP activity and suppresses tumourigenesis.Scientific reports · 2019Article
- The dysfunction of BP180/collagen XVII in keratinocytes promotes melanoma progression.Oncogene · 2019Article
- The direct binding of collagen XVII and collagen IV is disrupted by pemphigoid autoantibodies.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Immune Reaction to Type XVII Collagen Induces Intramolecular and Intermolecular Epitope Spreading in Experimental Bullous Pemphigoid Models.Frontiers in immunology · 2019Article
- Living with Keratinocytes.Stem cell reports · 2018Review
- Rare variant analysis in multiply affected families, association studies and functional analysis suggest a role for the ITGΒ4 gene in schizophrenia and bipolar disorder.Schizophrenia research · 2018Article
- Mammalian Plakins, Giant Cytolinkers: Versatile Biological Functions and Roles in Cancer.International journal of molecular sciences · 2018Review
- Spectraplakin family proteins - cytoskeletal crosslinkers with versatile roles.Journal of cell science · 2017Review
- Intermediate Filaments and the Plasma Membrane.Cold Spring Harbor perspectives in biology · 2017Review
- A hemidesmosomal protein regulates actin dynamics and traction forces in motile keratinocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Isoforms, structures, and functions of versatile spectraplakin MACF1.BMB reports · 2016Review
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Whole-genome sequencing identifies a homozygous deletion encompassing exons 17 to 23 of the integrin beta 4 gene in a Charolais calf with junctional epidermolysis bullosa.Genetics, selection, evolution : GSE · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In epidermal cells, the keratin cytoskeleton interacts with the elements in the basement membrane via a multimolecular junction called the hemidesmosome. A major component of the hemidesmosome plaque is the 230-kDa bullous pemphigoid autoantigen (BP230/BPAG1), which connects directly to the keratin-containing intermediate filaments of the cytoskeleton via its C terminus. A second bullous pemphigoid antigen of 180 kDa (BP180/BPAG2) is a type II transmembrane component of the hemidesmosome. Using yeast two-hybrid technology and recombinant proteins, we show that an N-terminal fragment of BP230 can bind directly to an N-terminal fragment of BP180. We have also explored the consequences of expression of the BP230 N terminus in 804G cells that assemble hemidesmosomes in vitro. Unexpectedly, this fragment disrupts the distribution of BP180 in transfected cells but has no apparent impact on the organization of endogenous BP230 and alpha6beta4 integrin. We propose that the BP230 N terminus competes with endogenous BP230 protein for BP180 binding and inhibits incorporation of BP180 into the cell surface at the site of the hemidesmosome. These data provide new insight into those interactions of the molecules of the hemidesmosome that are necessary for its function in integrating epithelial and connective tissue types.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.